This proposal outlines a plan to synthesize standard samples of a range of glycans as reference compounds in glycomics research. Cassia, LLC shall work in collaboration with the Paulson laboratory at The Scripps Research Institute to make these compounds commercially available to the research community.
The Specific Aims i nclude enzymatic synthesis of 20-32 glycans as either terminal fragments, or N- or O-linked glycans. In addition, these compounds shall be synthesized as "heavy" versions including 13C isotope labels to facilitate their use as internal standards for identification and quantitation of glycans using mass spectrometry. The glycans shall be characterized using NMR and mass spectrometry, and the compounds shall be supplied as standards for the National Institute of General Medical Sciences (NIGMS) microarray facility.
The specific aims i n Phase I of this Fast Track SBIR proposal are designed to supply the first wave of glycan products, and to begin to transfer the technology of enzymatic glycan synthesis from the Paulson laboratory to Cassia.
These aims set the foundation for synthesis of expanded glycan libraries in Phase II, and successful commercialization of a broad range of glycan reference compounds in Phase III Commercialization.

Agency
National Institute of Health (NIH)
Type
Small Business Innovation Research – Phase I (N43)
Project #
261201300051C-0-0-1
Application #
8758484
Study Section
Project Start
2013-09-12
Project End
2014-06-11
Budget Start
Budget End
Support Year
Fiscal Year
2013
Total Cost
$300,000
Indirect Cost
Name
Cassia, LLC
Department
Type
DUNS #
068373964
City
Del Mar
State
CA
Country
United States
Zip Code
92014