Data gaps exist in the assessment of human and environmental health largely due to the lack of ability to analyze large sample loads rapidly and cost effectively. Immunoassays can be used to measure toxic materials in the environment and for human monitoring because they are rapid, cost-effective, sensitive, specific and adaptable to laboratory or field situations. These assays can be used to measure potential exposure to these compounds in environmental samples such as soil and water, and in food. In the first 5 years of the Center, studies were conducted in Uruguay and elsewhere in South America that were previously cost prohibitive, thus providing an improved database from which to make informed decisions on human and environmental health. This project provides simple and reliable methods for residue analysis and a trained University staff to educate technicians in how to use these methods in the field. The Faculty of Chemistry, University of the Republic (Uruguay), has successfully acquired this methodology and is actively contributing to its further development. The University of California at Davis has special expertise in this area and represents an ideal training and technology transfer milieu to sponsor such a project. The project will continue to build regional capability for using immunoassays to monitor environmental contamination and to detect chemical residues in food products and drinking water according to the needs of the region. The University of the Republic in Montevideo will serve as the Regional Center of Excellence for training and technology transfer in this specialized area. In the Center renewal, we will increase participation by other countries in the region, especially Argentina, Chile, and Brazil, with additional collaborative projects in Peru. We will focus on laboratory training in modern molecular techniques, including ELISA and RT-PCR, to analyze drinking water quality, and on improved curricula for graduate training of professionals in toxicology, environmental toxicology, and public health. Participants will include environmental engineers, toxicologists, public health professionals, and epidemiologists, all of whom will benefit from regional-scale cooperation and improved training opportunities.

National Institute of Health (NIH)
Fogarty International Center (FIC)
International Research Training Grants (D43)
Project #
Application #
Study Section
Special Emphasis Panel (ZES1-SET-E (D4))
Program Officer
Rosenthal, Joshua
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Fiscal Year
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University of California Davis
Internal Medicine/Medicine
Schools of Medicine
United States
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Gutiérrez-Cacciabue, Dolores; Cid, Alicia G; Rajal, Verónica B (2016) How long can culturable bacteria and total DNA persist in environmental waters? The role of sunlight and solid particles. Sci Total Environ 539:494-502
Rossotti, Martín; Tabares, Sofía; Alfaya, Lucía et al. (2015) Streamlined method for parallel identification of single domain antibodies to membrane receptors on whole cells. Biochim Biophys Acta 1850:1397-404
Cruz, Mercedes Cecilia; Ruano, Gustavo; Wolf, Marcus et al. (2015) Plasma deposition of silver nanoparticles on ultrafiltration membranes: antibacterial and anti-biofouling properties. Chem Eng Res Des 94:524-537
Rossotti, Martin A; González-Techera, Andrés; Guarnaschelli, Julio et al. (2015) Increasing the potency of neutralizing single-domain antibodies by functionalization with a CD11b/CD18 binding domain. MAbs 7:820-8
Rossotti, Martín A; Pirez, Macarena; Gonzalez-Techera, Andres et al. (2015) Method for Sorting and Pairwise Selection of Nanobodies for the Development of Highly Sensitive Sandwich Immunoassays. Anal Chem 87:11907-14
Lassabe, Gabriel; Rossotti, Martín; González-Techera, Andrés et al. (2014) Shiga-like toxin B subunit of Escherichia coli as scaffold for high-avidity display of anti-immunocomplex peptides. Anal Chem 86:5541-6
Roegner, Amber F; Schirmer, Macarena Pírez; Puschner, Birgit et al. (2014) Rapid quantitative analysis of microcystins in raw surface waters with MALDI MS utilizing easily synthesized internal standards. Toxicon 78:94-102
Carlomagno, Mariana; Lassabe, Gabriel; Rossotti, Martín et al. (2014) Recombinant streptavidin nanopeptamer anti-immunocomplex assay for noncompetitive detection of small analytes. Anal Chem 86:10467-73
Moraga, Norma Beatriz; Poma, Hugo Ramiro; Amoroso, María Julia et al. (2014) Isolation and characterization of indigenous Streptomyces and Lentzea strains from soils containing boron compounds in Argentina. J Basic Microbiol 54:568-77
Gutiérrez-Cacciabue, Dolores; Teich, Ingrid; Poma, Hugo Ramiro et al. (2014) Strategies to optimize monitoring schemes of recreational waters from Salta, Argentina: a multivariate approach. Environ Monit Assess 186:8359-80

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