(Provided by the applicant) Abstract: This work will develop the capability to spatially detect a wide range of metabolites quantitatively and in real- time within a single cell. Few techniques are available to measure metabolite concentrations, although they are a key indicator of a cell's genomic expression, and the capability of metabolite flux measurements, resolved spatially and transiently, is heretofore unrealized. This project will provide these abilities to benefit several fields, including metabolomics, functional genomics, toxicology, cancer biology, metabolic engineering, and clinical diagnostics. The sensors will consist of single-walled carbon nanotubes which emit photostable near-infrared fluorescence which is highly environmentally sensitive. The nanotubes will be functionalized with ligands which bind to important metabolites and the binding event will be transduced by spectral shifts in the nanotube's emission. The investigator's group will introduce these nanotube sensors into live cells in order to answer important questions relevant to cancer biology and cell metabolism. Concomitantly, the team will work towards the rapid and real-time detection of clinically-relevant metabolites for early cancer diagnosis and the benchmarking of metastasis. We will also re-construct multiplexed metabolite data in order to conduct single- cell metabolomic fingerprinting. Public Health Relevance: Metabolites are small molecules produced in the body which may be used in the future to detect cancer at earlier stages, better understand the biology of cancer, and improve the development of important drugs. This project develops sensors using nanomaterials to detect many classes of metabolites within single cells and in human tissues.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
NIH Director’s New Innovator Awards (DP2)
Project #
1DP2HD075698-01
Application #
8358296
Study Section
Special Emphasis Panel (ZGM1-NDIA-C (01))
Program Officer
Grave, Gilman D
Project Start
2012-09-30
Project End
2017-08-31
Budget Start
2012-09-30
Budget End
2017-08-31
Support Year
1
Fiscal Year
2012
Total Cost
$2,743,500
Indirect Cost
$1,243,500
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065