The goal of this proposal is to determine the role of WD40 repeat containing protein 36 (WDR36) in the pathogenesis of adult-onset primary open-angle glaucoma (POAG). This will be accomplished using mainly the zebrafish model system. Glaucoma is a heterogeneous group of blinding diseases affecting 70 million people worldwide. The disease causes neurodegeneration of retinal ganglion cells and the optic nerve, resulting in visual field deficits. The first symptom experienced is vision loss, and by this time permanent nerve damage has already occurred. Adult-onset POAG is the most common form of glaucoma, and is very prevalent in the aging population. For example, 7% of Americans age 79 and over are affected by POAG, while 1.9% age 40 an over are affected. The genetics of POAG are complex, with multiple genes acting in concert to cause disease. Currently, only three genes have been determined to cause POAG. The most recently described is WDR36. The function of the gene and its role in disease pathogenesis are unknown, but sequence alignment and domain structure analysis have lead to the hypothesis that it is the homolog of the yeast nucleolar gene, utp21. Utp21 is required for biogenesis of mature ribosomes and is important for cell proliferation. Zebrafish with insertional mutations is both copies of wdr36 die during development, with small eyes, a small head, bilateral cataracts and a necrotic liver.
The first aim of this proposal will be to study the cellular functions of wdr36 in the zebrafish and in cell culture.
The second aim will be to characterize the role of wdr36 in development.
The third aim will be to mimic human adult-onset POAG by studying wdr36 heterozygous mutant fish during aging. Understanding the genes, such as WDR36, involved in the complex genetics of POAG will aid in the development of novel screening and treatment options for patients at risk or suffering from glaucoma. ? ? ?
| Skarie, Jonathan M; Link, Brian A (2009) FoxC1 is essential for vascular basement membrane integrity and hyaloid vessel morphogenesis. Invest Ophthalmol Vis Sci 50:5026-34 |
| Skarie, Jonathan M; Link, Brian A (2008) The primary open-angle glaucoma gene WDR36 functions in ribosomal RNA processing and interacts with the p53 stress-response pathway. Hum Mol Genet 17:2474-85 |
| Berry, Fred B; Skarie, Jonathan M; Mirzayans, Farideh et al. (2008) FOXC1 is required for cell viability and resistance to oxidative stress in the eye through the transcriptional regulation of FOXO1A. Hum Mol Genet 17:490-505 |
