Viral infections are associated with several human malignancies, yet the role of viral infections in the central nervous system (CNS) tumors remains unclear. Although human cytomegalovirus (HCMV) has been associated with glioblastoma multiforme (GBM), the mechanisms of pathogenesis remain to be determined. GBM is the most common primary malignancy in the CNS, and unfortunately, it is also one of the most devastating. Novel approaches to studying this disease are paramount for the advancement in treatment options for patients with this dismal disease. Using robust next generation sequencing technology, this project aims to provide insight into HCMV biology related to GBMs. In addition, investigation of the tumor microenvironment for HCMV specific host cellular gene changes will lead to the discovery of pathways in which HCMV dysregulation promotes GBM pathology. The overarching hypothesis for this project is that HCMV contributes to GBM pathogenesis through modulation of the tumor microenvironment.

Public Health Relevance

Glioblastoma multiforme (GBM) is one of the most devastating cancers in humans. With an average survival time of just over one year, there is a significant need for advanced therapeutic options and a more in-depth understanding of its biology. Human cytomegalovirus (HCMV) has been linked to GBMs, but the underlying mechanisms by which HCMV promotes GBM pathogenesis are currently unclear. An understanding of HCMV and GBMs is not only important for understanding the disease process but also to guide the development of preventative and/or therapeutic approaches including vaccines and anti-virals.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Individual Predoctoral NRSA for M.D./Ph.D. Fellowships (ADAMHA) (F30)
Project #
1F30CA177267-01
Application #
8527109
Study Section
Special Emphasis Panel (ZRG1-F13-C (20))
Program Officer
Damico, Mark W
Project Start
2013-04-01
Project End
2018-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
1
Fiscal Year
2013
Total Cost
$26,412
Indirect Cost
Name
Tulane University
Department
Pathology
Type
Schools of Medicine
DUNS #
053785812
City
New Orleans
State
LA
Country
United States
Zip Code
70118
Lin, Zhen; Swan, Kenneth; Zhang, Xin et al. (2016) Secreted Oral Epithelial Cell Membrane Vesicles Induce Epstein-Barr Virus Reactivation in Latently Infected B Cells. J Virol 90:3469-79
Strong, Michael J; Blanchard 4th, Eugene; Lin, Zhen et al. (2016) A comprehensive next generation sequencing-based virome assessment in brain tissue suggests no major virus - tumor association. Acta Neuropathol Commun 4:71
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Strong, Michael J; Rosenlof, Trevor; Padmanabha, Siddhartha et al. (2015) Treatment of recurrent metastatic uterine leiomyosarcoma of the spine: a multimodality approach using resection, radiosurgery, and chemotherapy. J Neurosurg Spine :1-6
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Cao, Subing; Moss, Walter; O'Grady, Tina et al. (2015) New Noncoding Lytic Transcripts Derived from the Epstein-Barr Virus Latency Origin of Replication, oriP, Are Hyperedited, Bind the Paraspeckle Protein, NONO/p54nrb, and Support Viral Lytic Transcription. J Virol 89:7120-32
Strong, Michael J; Garces, Juanita; Tang, Wendell et al. (2015) Benign Sacral Metastatic Meningioma: A Rare Entity. Ochsner J 15:200-2
Strong, Michael J; Baddoo, Melody; Nanbo, Asuka et al. (2014) Comprehensive high-throughput RNA sequencing analysis reveals contamination of multiple nasopharyngeal carcinoma cell lines with HeLa cell genomes. J Virol 88:10696-704
Thompson, Eric M; Strong, Michael J; Warren, Garth et al. (2014) Clinical significance of imaging and histological characteristics of filum terminale in tethered cord syndrome. J Neurosurg Pediatr 13:255-9

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