In the past 25 years, little improvement has been made in patient survival after a diagnosis of glioblastoma multiforme (GBM). The robust induction of angiogenesis, extreme resistance to radiation, and a highly aggressive, invasive phenotype make GBM one of the most lethal and difficult human cancers to treat. Recently, a mesenchymal subtype of GBMs has been identified that displays the most infiltrative behavior, is the most resistant to standard therapies, and expresses markers, such as a secreted protein YKL-40, which correlate with the worst patient prognosis. We have identified that two proinflammatory cytokines, interleukin-1? (IL-1?) and oncostatin M (OSM), are specifically overexpressed in patients with mesenchymal GBM. Our data indicates that IL-1? and OSM synergize to support GBM progression through a novel RelB/p50-canonical pathway, which to date has only been proposed to function in cells expressing high levels of RelB. Our preliminary studies have identified two groups of RelB/p50-dependent genes in GBM cells and astrocytes: conserved genes (activated in both GBM and astrocytes) and aberrantly-activated genes (activated in GBM but suppressed in astrocytes). The conserved genes include markers of the mesenchymal GBM subtype and intriguing novel genes postulated to regulate GBM progression. The aberrantly activated genes encode many cytokines, including IL-1, which suggests the existence of RelB-driven feedforward cytokine loop fueling chronic inflammation in GBM. Importantly, the aberrantly-activated genes have previously been shown to undergo RelB-dependent reprograming by epigenetic silencing in macrophages, which requires histone deacetylase SIRT1. Our preliminary studies have found that one allele of the sirt1 gene is deleted in 75% of GBM tumors, which may explain the lack of RelB-dependent epigenetic silencing in GBM. Thus, we hypothesize that RelB/p50 activation drives gene expression programs which induced both chronic inflammation and GBM aggressiveness.

Public Health Relevance

Glioblastoma Multiforme (GBM) is the most lethal primary malignant brain tumor, which derives much of its aggressive behavior from the inflammatory microenvironment. We have identified a novel NF-?B signaling dimer, RelB/p50, previously thought only to be active in hematopoietic cells, to be active in GBM and primary astrocytes, where this dimer controls the production of key genes in GBM progression and drives an aberrant feed-forward inflammatory program in GBM cells, but not primary astrocytes. This study aims to further characterize this pathway, and evaluate its effects on GBM progression and chronic inflammation.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Individual Predoctoral NRSA for M.D./Ph.D. Fellowships (ADAMHA) (F30)
Project #
1F30CA200252-01A1
Application #
9050158
Study Section
Special Emphasis Panel ()
Program Officer
Damico, Mark W
Project Start
2016-03-25
Project End
2021-03-24
Budget Start
2016-03-25
Budget End
2017-03-24
Support Year
1
Fiscal Year
2016
Total Cost
$35,096
Indirect Cost
Name
Virginia Commonwealth University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
105300446
City
Richmond
State
VA
Country
United States
Zip Code
23298