Our long-term goal is to translate the use of novel, diagnostic plasma biomarkers for alcohol intake developed in non-human primates into a convenient clinical diagnostic tool for human samples. The specific objective of this proposal is to reproduce the differentiation between drinking and naove phenotypes that we have previously demonstrated in non-human primates with the more clinically practical, high throughput multiple reaction monitoring (MRM) technology, and to then translate the use of this technology into the clinical realm with human samples. The central hypothesis is that our diagnostic panel of plasma biomarkers will be able to differentiate drinking versus naove status in human samples via MRM with high sensitivity, specificity, and reproducibility. We base this hypothesis, in part, on the high analytical precision and robustness of MRM technology and on our strong preliminary evidence in non-human primates that a panel of plasma proteins can provide nearly 100% specificity and sensitivity in distinguishing between drinking and naove phenotypes. The rationale for the proposed research is that we can use MRM quantitation, in conjunction with our panel of plasma biomarkers, as a platform to provide objective diagnostic technology that is currently lacking and that would improve our understanding and treatment of excessive alcohol intake.

Public Health Relevance

We plan to validate a highly accurate biomarker panel able to differentiate alcohol intake in non-human primates and translate it for use in humans via superior proteomic technology.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31AA019891-01
Application #
8004756
Study Section
Health Services Research Review Subcommittee (AA)
Program Officer
Jung, Kathy
Project Start
2010-09-01
Project End
2013-08-31
Budget Start
2010-09-01
Budget End
2011-08-31
Support Year
1
Fiscal Year
2010
Total Cost
$27,271
Indirect Cost
Name
Pennsylvania State University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
129348186
City
Hershey
State
PA
Country
United States
Zip Code
17033