The aging population poses significant social and health challenges. Of particular importance in the study of molecular and social aspects of senescence is achieving a comprehensive understanding of behavioral and neurobiological processes that occur as part of both normal aging and disease. The development of a social invertebrate model of aging, the ant Pheidole dentata, presents multiple advantages in the analysis of aging. 1. Like humans, ants are highly dependent on social interactions, and we can experimentally manipulate the social environment in ants to rapidly assess its influence on molecular, neural and behavioral aspects of aging;2. Research on P. dentata, the "fruit fly" of sociobiology, has yielded significant progress in understanding behavioral and neurobiological development in this species;and 3. Workers undergo behavioral development within 20 days of eclosion and have a lifespan of only four and a half months, making them useful models to explore patterns of neurodegeneration and cognitive decline within an accelerated timeframe. The development of our new social model of aging will illuminate how social interactions mediate neural and behavioral decline and determine if serotonin, which is significant in behavioral maturation in ants and cognitive functioning in humans afflicted with neurodegenerative disease, can rescue aging phenotypes. The proposed research examines if age-associated behavioral decline is mediated by neurodegenerative cell death and/or changes in brain serotonin according to social context. We predict: 1) Behavioral efficacy will decline in old-age workers. 2) Brain apoptosis will be high throughout behavioral development and decline with increasing age;and 3) Serotonin levels will decrease in senescing individuals and will decline more rapidly in individuals in a deprived social environment. Furthermore, pharmacological alteration of brain serotonin using both a serotonin precursor and inhibitor will determine if serotonin is sufficient to rescue behavioral decline. Our findings will expand our understanding of the relationship between age-related behavior and senescence, and examine how sociality and neuromodulators may mitigate the sensory and motor consequences of neurodegeneration and aging. We have three specific aims:
Specific Aim 1 : To characterize senescence-associated behavioral changes by quantifying olfactory sensitivity and motor performance in P. dentata workers.
Specific Aim 2 : To immunohistochemically localize and quantify neuronal apoptosis.
Specific Aim 3 : To determine whether serotonin can rescue age-related decline in sensory and motor function in a social context by HPLC analysis and pharmacological treatments.

Public Health Relevance

Human aging is influenced by social interactions. We have developed a novel insect model system to examine how social behavior and the neurotransmitter serotonin may regulate age-related declines in behavior. The common occurrence of serotonin and great similarity in molecular events of neurodegeneration in insects and humans allows us to efficiently examine the effect of brain chemistry and the social environment on cognitive decline and neural processes through experimental manipulation.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31AG041589-02
Application #
8516336
Study Section
Special Emphasis Panel (ZRG1-F02B-M (20))
Program Officer
Chen, Wen G
Project Start
2012-09-01
Project End
2015-08-31
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
2
Fiscal Year
2013
Total Cost
$29,697
Indirect Cost
Name
Boston University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
049435266
City
Boston
State
MA
Country
United States
Zip Code
02215
Giraldo, Ysabel Milton; Traniello, James F A (2014) Worker senescence and the sociobiology of aging in ants. Behav Ecol Sociobiol 68:1901-1919