Children with Down syndrome (DS) have an unusually high incidence of acute megakaryoblastic leukemia (DS-AMKL), a malignancy that results in increased numbers of abnormal megakaryoblasts, the platelet-producing cells in the blood. Studies have shown that mutations in GATA1, an essential hematopoietic transcription factor, are present in all cases of DS-AMKL as well as in the pre-leukemia of DS named transient myeloproliferative disorder (TMD). Nearly 20% of infants with TMD develop DS-AMKL within two years. Recent next-generation sequencing studies suggest that trisomy 21, the cause of DS, and a GATA1 mutation are sufficient for TMD, but that an additional genetic event is necessary for the transformation from TMD to DS- AMKL. Of note, more than half of patients with DS-AMKL have mutations in genes in the subunits of the cohesin complex, suggesting that a cohesin defect is a major driver of leukemic progression. The high incidence of cohesin mutations in DS-AMKL is in marked contrast to the 10% incidence in other hematologic malignancies, even in non-DS AMKL. Cohesin is a ring-shaped complex that regulates gene expression by forming chromatin loops between gene promoters and enhancer elements. Recent studies have shown that cohesin mutations lead to enhanced stem cell activity and myeloproliferative disease, and that they cooperate with FLT3-ITD to induce leukemia in vivo. How cohesin mutations contribute to DS-AMKL, however, has not been explored.
My research aims to determine how trisomy 21 and GATA1 mutations cooperate with cohesin mutations to cause DS-AMKL.
My specific aims are to: 1) Determine the contribution of cohesin mutations to leukemic transformation in vitro in a trisomy 21/ GATA1 mutant background; 2) Elucidate whether cohesin loss in the context of DS and a GATA1 mutation is sufficient to induce aberrant hematopoiesis and/or AMKL; and 3) Identify molecular targets of cohesin responsible for progression to leukemia. These studies will provide us with a better understanding of the mechanism behind DS-AMKL and likely lead to the identification of new therapeutic targets for this malignancy. My sponsor Dr. Crispino and I have jointly developed a powerful mentoring plan that, along with this F31 award, will enable my successful transition to a post-doctoral researcher and eventually to an independent principal investigator.

Public Health Relevance

Children with Down syndrome (DS) are 500 times more likely to develop acute megakaryoblastic leukemia (AMKL). This leukemia is caused by the combination of a GATA1 mutation, trisomy 21, and a third mutation, which, in more than half the cases, is in one of the genes that make up the cohesin complex. My research will help us understand how cohesin mutations cause cancer and may lead to improvements in the treatments for patients with cohesin mutated AMKL and other subtypes of acute myeloid leukemia.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31CA216976-02
Application #
9539160
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Radaev, Sergey
Project Start
2017-09-01
Project End
2019-12-31
Budget Start
2018-09-01
Budget End
2019-08-31
Support Year
2
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Northwestern University at Chicago
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611