Endomorphin-2 (Tyr-Pro-Phe-NH2) is an endogenous morphine-like substance which binds to the mu opiate receptor with high affinity and selectivity. Endomorphin-2-like immunoreactivity has been demonstrated in the medulla and superficial layers of the dorsal horn in the spinal cord and in primary afferents (reprint enclosed), suggesting a role for this peptide in pain transmission. This program will test the hypothesis that endomorphin-2 is decreased in these areas after inflammation and chronic constriction injury. This reduction in endomorphin is postulated to produce a loss of inhibition of excitatory transmitters and contribute to the hyperalgesia known to occur in these pathological states.
In Specific Aims 1 and 2, a unilateral inflammatory response or chronic constriction injury will be induced. Immunocytochemistry will be used to test the hypothesis that endomorphin-2-like immunoreactivity is reduced on the inflamed or injured side relative to the contralateral side and sham controls.
Specific Aim 3 will examine the ability of exogenously administered endomorphin-2 to inhibit hyperalgesia and allodynia induced by administration of complete Freund's adjuvant (CFA).

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31DA005968-01
Application #
6013802
Study Section
Special Emphasis Panel (ZDA1-MXS-M (20))
Program Officer
Babecki, Beth
Project Start
1999-07-15
Project End
Budget Start
1999-07-15
Budget End
2000-07-14
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Tulane University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
City
New Orleans
State
LA
Country
United States
Zip Code
70118