Nanotechnology applications are being incorporated into our daily lives, but the safety of nanomaterials usage still awaits thorough assessment. Currently, little is known about nanomaterial-biological interactions, and to bridge this gap, I propose using embryonic zebrafish as a rapid, in vivo system to investigate the activity of nanomaterials at the molecular level. High-purity, ligand-functionalized silver nanoparticles (AgNPs) can be precisely engineered to custom-manipulate physicochemical properties. I hypothesize that the biological activity of nanomaterials is dependent upon primary particle size, size distribution, chemical composition of surface groups, surface charge and state of agglomeration. To test this hypothesis, I will collect toxicity data including morbidity and mortality dose response, uptake concentration, and nanoparticle exposure-induced changes in gene expression. Additionally, by exposing embryonic zebrafish to silver nanoparticles (AgNPs) engineered to exhibit highly specific physicochemical properties, I will define which properties are responsible for causing specific biological effects. All data will be recorded in the Nanomaterial-Biological Interactions (NBI) knowledge base. The NBI knowledgebase serves as a warehouse for annotated data on nanomaterial characterization, synthesis methods, and nanomaterial-biological interactions defined at multiple levels of biological organization. The data I submit to the NBI knowledgebase will facilitate identification of key data for predicting the biological effects of nanomaterial exposure based on physicochemical properties.

Public Health Relevance

Structure-activity based prediction of health hazard potential of nanomaterials should be in place during technology development. Given that nanomaterials are becoming pervasive, it is imperative that science rapidly develops structure-biological activity relationships for new and emerging classes of nanomaterials. This will serve to both ensure human safety of the technology and minimize the gross inefficiency of blindly developing inappropriate uses of nanomaterials for biomedical and consumer products.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31ES019445-03
Application #
8304353
Study Section
Special Emphasis Panel (ZRG1-IMST-D (29))
Program Officer
Humble, Michael C
Project Start
2010-08-01
Project End
2013-07-31
Budget Start
2012-07-14
Budget End
2013-07-13
Support Year
3
Fiscal Year
2012
Total Cost
$33,883
Indirect Cost
Name
Oregon State University
Department
Public Health & Prev Medicine
Type
Schools of Earth Sciences/Natur
DUNS #
053599908
City
Corvallis
State
OR
Country
United States
Zip Code
97339
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Levard, Clement; Hotze, Ernest M; Colman, Benjamin P et al. (2013) Sulfidation of silver nanoparticles: natural antidote to their toxicity. Environ Sci Technol 47:13440-8
Kim, Ki-Tae; Truong, Lisa; Wehmas, Leah et al. (2013) Silver nanoparticle toxicity in the embryonic zebrafish is governed by particle dispersion and ionic environment. Nanotechnology 24:115101
Truong, Lisa; Tilton, Susan C; Zaikova, Tatiana et al. (2013) Surface functionalities of gold nanoparticles impact embryonic gene expression responses. Nanotoxicology 7:192-201
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Truong, Lisa; Zaikova, Tatiana; Richman, Erik K et al. (2012) Media ionic strength impacts embryonic responses to engineered nanoparticle exposure. Nanotoxicology 6:691-9
Mandrell, David; Truong, Lisa; Jephson, Caleb et al. (2012) Automated zebrafish chorion removal and single embryo placement: optimizing throughput of zebrafish developmental toxicity screens. J Lab Autom 17:66-74
Truong, Lisa; Harper, Stacey L; Tanguay, Robert L (2011) Evaluation of embryotoxicity using the zebrafish model. Methods Mol Biol 691:271-9
Truong, Lisa; Moody, Ian S; Stankus, Dylan P et al. (2011) Differential stability of lead sulfide nanoparticles influences biological responses in embryonic zebrafish. Arch Toxicol 85:787-98