The degradation of various constituents of the extracellular matrix including, heparan sulfate proteoglycans, represents a critical step in the process of cancer invasion and metastasis. Heparanase, an endo-p-D- glycosidase which degrades HS, has emerged as a promising target for the development of anti-cancer treatment because of its pro-metastatic, pro-inflammatory, and pro-angiogenic activity. Therefore we sought to utilize a novel enzymatic approach to probe the specificity of heparanase and its inhibitor.
|Peterson, Sherket B; Liu, Jian (2013) Multi-faceted substrate specificity of heparanase. Matrix Biol 32:223-7|
|Peterson, Sherket; Liu, Jian (2012) Deciphering mode of action of heparanase using structurally defined oligosaccharides. J Biol Chem 287:34836-43|
|Peterson, Sherket B; Liu, Jian (2010) Unraveling the specificity of heparanase utilizing synthetic substrates. J Biol Chem 285:14504-13|