Cervical remodeling is an essential part of normal pregnancy and is characterized by softening, shortening and dilation to allow for delivery at term. Errors in timing of these events may be associated with preterm birth. Currently, two of the main tools for assessment of the cervix include a cervical length measurement and digital palpation of `softness'. While the former is quantitative, the `softness' is subjectively determine by giving it a score of `soft', `medium', or `?rm'. The primary goal of this research is to develp a reliable, noninvasive method to quantitatively assess the structural integrity of the uterine cervi during pregnancy. Our preliminary studies show that Shear Wave Speed (SWS) estimation can objectively assess the softness of the cervix and that cystic structures perturb these estimates and should be avoided. However, other strong isolated scatterers such as blood vessels or dense collagen structures may also in?uence SWS estimates. We can use echo signal coherence to help guide location se- lection to avoid these structures for more robust SWS estimates. We have completed studies showing feasibility of SWS estimation in ex vivo hysterectomy specimens and in vivo third trimester pregnant subjects and demonstrated that the approach has merit. For the ?rst portion of this proposed investigation, ultrasound data has already been collected from a previous hysterectomy study and all samples have been ?xed in formalin. We plan to complete echo signal coherence analysis in order to identify collagen structures spatially that may in?uence SWS measurement and validate with microscopy of the ?xed samples. The second portion of this proposed investigation will test reproducibility and sensitivity of SWS estimation in vivo during the ?rst and second trimester of pregnancy. We will measure SWS in women undergoing cervical ripening procedures for early termination of pregnancy. Measurements will be made before and 4 hours after a cervical ripening agent is administered. Pre- and post-ripening SWS estimates will be compared within each woman and the 1st and 2nd trimester groups will be compared to the (nonpregnant) hysterectomy data and the 3rd trimester data already acquired. This proposal lays the foundation for understanding how cervical structural integrity changes throughout pregnancy which is essential for understanding normal and abnormal pregnancy. In addition, a full understanding of how structure in?uences quantitative ultrasound measure- ments will guide future in vivo study. The training plan includes ?eld work to be performed in Utah, in which the PI will be immersed in the clinical environment and have more involvement in management of 1st and 2nd trimester study.

Public Health Relevance

This is a proposal to develop quantitative ultrasound measurements to objectively quantify cervical structural integrity (changes in collagen microstructure and softness) in the in vivo pregnant cervix. Cervical softening occurs early on in pregnancy and clinicians recognize this as a critical measurement for risk assessment of preterm birth, but have no means of quantitatively assessing it. Preliminary results demonstrate that Quantitative Ultrasound (QUS) can objectively measure changes in cervical softness, but a more clear understanding of the changes during pregnancy is needed to create a predictive model of abnormal cervical softening. This study lays the groundwork for that understanding.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31HD082911-02
Application #
9242510
Study Section
Special Emphasis Panel (ZRG1-F15-P (20)L)
Program Officer
Koso-Thomas, Marion
Project Start
2016-04-01
Project End
2018-03-31
Budget Start
2017-04-01
Budget End
2018-03-31
Support Year
2
Fiscal Year
2017
Total Cost
$31,913
Indirect Cost
Name
University of Wisconsin Madison
Department
Physics
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Carlson, Lindsey C; Hall, Timothy J; Rosado-Mendez, Ivan M et al. (2017) Detection of Changes in Cervical Softness Using Shear Wave Speed in Early versus Late Pregnancy: An in Vivo Cross-Sectional Study. Ultrasound Med Biol :