Breast cancer is the second leading cause of cancer-related death and second most frequently diagnosed cancer in women in the United States1. Breast conserving surgery with radiation has emerged as the preferred method for treating breast cancer while preserving breast tissue and without compromising survival rates. Local recurrence following partial mastectomy is strongly correlated to the surgical margin2-4, but despite evaluation using a combination of intraoperative examination and post-operative histopathology, 40% of patients require surgical re-excision as a result of positive or close margins5, 6. Two-photon microscopy (TPM) has demonstrated significant resolution, molecular specificity, and light efficiency advantages over conventional white-light and fluorescence microscopes for imaging endogenous and exogenous contrast both in vivo and ex vivo7-9. However, scattering tissue severely degrades two-photon signals and resolution, limiting the fundamental imaging depth of TPM to ~100 The proposal emphasizes the development of an imaging system that combines optical coherence tomography with two-photon microscopy for biomarker specific imaging of surgical margins for breast cancer detection. By combing this novel multimodal system with wavefront correction methods, we can enhance the detection depth, sensitivity, and specificity to achieve rapid intraoperative assessment of tumor margins as a means of reducing the morbidity and mortality associated with breast cancer.Public Health Relevance
| Tao, Yuankai K; Shen, Dejun; Sheikine, Yuri et al. (2014) Assessment of breast pathologies using nonlinear microscopy. Proc Natl Acad Sci U S A 111:15304-9 |
