The obesity epidemic is associated with excessive intake of calorie dense, palatable foods and fluids. Oral and postoral chemical signals are integrated in the central nervous system to adjust food and fluid selection, control meal size, and establish taste preferences. Yet little is known about precisely how these signals are integrated to change intake behavior. Progress has been hampered by the lack of available sensitive techniques that probe postoral chemosensory signals and link those to intake behaviors. Recently, chemoreceptors and associated signaling molecules involved in taste transduction (e.g., T1Rs, T2Rs, ? -gustducin) have been discovered in postoral GI cells, suggesting the interesting possibility that taste properties are among the information encoded in ascending postoral signals and may directly access and impact matching chemosensory signals arising from the oral cavity. Considering oral and postoral sensory signals ascend the central nervous system in roughly parallel pathways, it is especially surprising then that very little experimental attention has been paid to direct influence of postoral signals on primary taste sensory processing. As such, the presence of a common sensory system provides a framework for probing chemospecific influences of postoral signals on well characterized taste-guided behaviors. The present proposal seeks support to begin the psychophysical assessment of chemospecific influences of postoral signals on taste responsiveness in a rat model. Two taste-specific, and complementary, behavioral techniques will be used to assess the effects of intraduodenal chemical infusions on (a) hedonic evaluation of and (b) sensitivity to, independent of the hedonic valence of the stimulus, an oral taste stimulus.
Specific Aim 1 tests the hypothesis that postgastric signals influence the hedonic evaluation of an oral taste stimulus in a chemospecific manner in the taste reactivity test.
Specific Aim 2 tests the hypothesis that postgastric signals influence oral taste sensitivity in a chemospecific manner in the two response operant taste signal detection task. Not only will applying these psychophysical techniques identify novel, and potentially, important types of primary sensory integration, it will afford me training in new behavioral approaches and data analyses (e.g., psychometric functions) and expose me to a new area of conceptual expertise centered on gustatory function. Such psychophysical testing of GI taste signals through their specific impact on oral taste processing will direct future work on the underlying neural mechanisms, with the ultimate goal of identifying potential targets and strategies to augment sensory feedback crucial to the control of food intake.

Public Health Relevance

Overweight and obesity with their associated health consequences, such as diabetes and cardiovascular disease, are now among the leading causes of preventable death in the United States. The concomitant increase in consumption of highly palatable and weakly satiating foods places special emphasis on understanding the critical integration of early food-related sensory signals arising from the oral cavity and gastrointestinal tract during the meal. The research proposed here seeks to probe direct, chemosensory- specific effects of postoral chemical signals on taste evaluation and sensitivity, with the long term goal of identifying foods and other interventions that potentiate gastrointestinal negative feedback on taste processing.

National Institute of Health (NIH)
National Institute on Deafness and Other Communication Disorders (NIDCD)
Postdoctoral Individual National Research Service Award (F32)
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Special Emphasis Panel (ZDC1)
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Sklare, Dan
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Florida State University
Schools of Arts and Sciences
United States
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Schier, Lindsey A; Blonde, Ginger D; Spector, Alan C (2016) Bilateral lesions in a specific subregion of posterior insular cortex impair conditioned taste aversion expression in rats. J Comp Neurol 524:54-73
Schier, Lindsey A; Spector, Alan C (2016) Behavioral Evidence for More than One Taste Signaling Pathway for Sugars in Rats. J Neurosci 36:113-24
Schier, Lindsey A; Hashimoto, Koji; Bales, Michelle B et al. (2014) High-resolution lesion-mapping strategy links a hot spot in rat insular cortex with impaired expression of taste aversion learning. Proc Natl Acad Sci U S A 111:1162-7