The field of proteomics is important as a basic science - probing the biochemical basis of life - and for a myriad of clinical applications, ranging from drug discovery to assays for gene expression. Proteomics is currently limited by a lack of high-throughput methods to identify proteins in complex samples. A novel combination of the electrowetting-on-dielectric (EWOD) fluid handling technique and matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) is proposed in response to this challenge. This work will be pursued with two primary goals: (1) to establish the merits of EWOD-based arrays of protein samples for high-throughput analysis with MALDI-MS, and (2) to enhance the functionality of this technology by demonstrating a variety of in-line processing steps, including sample decontamination and proteolytic digestion. If successful, this will be a major advance over existing technologies, which do not have the capacity to combine high-throughput arrays with sample processing.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32EB003696-02
Application #
6875623
Study Section
Special Emphasis Panel (ZRG1-F04 (20))
Program Officer
Khachaturian, Henry
Project Start
2004-04-01
Project End
2005-06-30
Budget Start
2005-04-01
Budget End
2005-06-30
Support Year
2
Fiscal Year
2005
Total Cost
$13,199
Indirect Cost
Name
University of California Los Angeles
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095