The objective of this research is to determine how expression of c-type cytochromes is regulated. These hemoproteins are vital for energy- producing electron transport chains of eukaryotes and many prokaryotes, and defects in their production have been associated with mammalian mitochondrial myopathies. To understand how production of these proteins is controlled, this fellowship will focus on cytochrome c2 of the facultative phototroph Rhodobacter sphaeroides. In this organism, cytochrome c2 is a multi-functional electron carrier that is utilized in different energy-producing transport chains depending upon the metabolic state of the bacterium. The proposed experiments will address how expression of the cytochrome c2 gene is regulated under anaerobic conditions, in response to the alternative electron acceptor dimethyl sulfoxide during anaerobic respiration, and in transitions between various aerobic and anaerobic lifestyles. The results of these studies will provide a detailed picture of the new regulatory hierarchies that control expression of multi-functional electron carriers such as cytochrome c2.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32GM020344-03
Application #
6518831
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Tompkins, Laurie
Project Start
2000-03-01
Project End
Budget Start
2002-03-01
Budget End
2002-08-31
Support Year
3
Fiscal Year
2002
Total Cost
$25,058
Indirect Cost
Name
University of Wisconsin Madison
Department
Microbiology/Immun/Virology
Type
Schools of Earth Sciences/Natur
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715