Abstract

Streptococcus pyogenes (group A streptococcus or GA) is the causative agent of a number of human diseases, including pharyngitis, rheumatic fever, streptococcal toxic shock syndrome and necrotizing fasciitis. Our laboratory recently showed that mucRS is a negative regulatory of three importance virulence factor in GS.
The aim of this proposal is to determine the molecular mechanisms involved in mucRS-mediated regulation of gene expression and how this relates in mucRS-mediated regulation of gene expression and how this relates to virulence in GAS. I will (i) characterize the mechanism(s) of repression of MucR, (ii) examine the role of phosphorylation of MucR, (iii) analyze the effect of MucS activity on MucR and (iv) determine the effect of a defined set of environmental conditions of MucRS activity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32GM020420-01
Application #
6071275
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Program Officer
Wolfe, Paul B
Project Start
2000-05-01
Project End
Budget Start
2000-05-01
Budget End
2001-04-30
Support Year
1
Fiscal Year
2000
Total Cost
$39,232
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Urology
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Heath, A; Miller, A; DiRita, V J et al. (2001) Identification of a major, CsrRS-regulated secreted protein of Group A streptococcus. Microb Pathog 31:81-9
Miller, A A; Engleberg, N C; DiRita, V J (2001) Repression of virulence genes by phosphorylation-dependent oligomerization of CsrR at target promoters in S. pyogenes. Mol Microbiol 40:976-90