DNA repair and recombination is crucial for the fidelity of DNA replication. The RecQ family of DMAhelicases is required for this process and in humans, mutations in these genes leads to the Bloom's,Werner's, and Rothmund-Thomson syndromes. All of these diseases are fundamentally characterized by apredisposition to cancer due to chromosomal loss and rearrangement. In yeast, the Sgs1 protein, is the onlyhomologue to the human RecQ family of DNA helicases. Mutations in this protein recapitulate the affectsobserved with the human diseases suggesting that its functions have been conserved to humans. Due tothe vast ease of doing genetics, cellular biology, and molecular biochemistry in yeast, this model organism isideal to study this family of DNA helicases. Outlined here, I plan to further characterize the role of the Sgs1protein in the DNA repair pathway and to find and characterize additional interacting proteins to Sgs1. Usingtechniques developed in the Rothstein laboratory, I will be able to further understand the mechanism of howthe Sgs1 protein leads to cancer development in higher eukaryotes.
