This major goals of this research proposal are 1) to compare engraftment of adult human G-CSF mobilized peripheral blood CD34+ cells after intravenous, intracoronary or intracardiac administration techniques, 2) to quantify infarct neovascularization and functional recovery after CD34+ cell administration (administered by the best technique above) and 3) compare the subsequent engraftment, transdifferentiation and functional improvement following intramyocardial injection of various cell populations used in cellular cardiac repair, including multipotent adult progenitor cells (MAPCs), unfractionated bone marrow mononuclear cells (BMCs) and skeletal myoblasts (SkMBs). This research protocol should elucidate the importance of infarct neovascularization with respect to subsequent cellular engraftment and transdifferentiation, a significant obstacle thus far in the rapidly progressing field of cellular cardiomyoplasty. An additional beneficial readout of this experiment is a functional and histologic comparison of multiple cell lineages currently being used in the field.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HL082134-01
Application #
6994564
Study Section
Special Emphasis Panel (ZRG1-DIG-B (21))
Program Officer
Meadows, Tawanna
Project Start
2005-07-22
Project End
2008-07-21
Budget Start
2005-07-22
Budget End
2006-07-21
Support Year
1
Fiscal Year
2005
Total Cost
$65,729
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Surgery
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
McCue, Jonathan D; Swingen, Cory; Feldberg, Tanya et al. (2008) The real estate of myoblast cardiac transplantation: negative remodeling is associated with location. J Heart Lung Transplant 27:116-23