Given the ubiquitous nature of K+ channels, it seems likely that these channels play important roles in the normal function of all cells. Thus, it is important to determine the purpose of individual channels in specific cells and tissues of the body. As a step toward this goal, I plan to generate a null mutant ('knock-out') mouse that lacks the Kv1.3 (n type), voltage-gated K+channel. This will be done using techniques, such as homologous recombination that are already available and that have previously been used in this laboratory to make a Kv3.1 (l type) K+ channel-deficient mouse. The ultimate goal is to determine the phenotype of Kv1.3-deficient mice using behavioral, morphologic, physiologic and immunologic techniques. Although little is currently known about the location or the role of the Kv1.3 channel in animals, it has been identified as the n type channel found in various cells of the human and mouse immune systems, including T lymphocytes. Previous studies of the Kv1.3 channel in T lymphocytes led to the interesting suggestion that it is involved in mitogen-induced activation of T cells. Some evidence of its expression in the brain has also been found. Thus, my initial studies of the mutant mice will focus on the immune and nervous systems. I expect to gain information regarding the role of the Kv1.3 K+ channel in the development and function of these systems. Information regarding a role of this channel in disease may also be derived from this study.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32NS010225-02
Application #
2445688
Study Section
Special Emphasis Panel (ZRG1-NEUB-1 (01))
Program Officer
Baughman, Robert W
Project Start
1997-07-01
Project End
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
2
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390