This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. SUBPROJECT DESCRIPTION The Molecular biology (MBTE) core facility, which is funded through bridge funding, continues to provide research support to investigators through tools, techniques and services required to perform molecular and cellular biomedical research. We are requesting continued bridge funding support from the RCMI Program for the continued functioning of the MBTE core facility, while the RCMI renewal application is being prepared for submission in May 2011. The MBTE core facility will be part of the new RCMI competitive renewal application. The MBTE core facility resources are vital to the goals of our new RCMI program focusing on advanced molecular analysis of genomes, epigenomes and proteomes, which will assist in the understanding of disease pathogenesis and promotion of drug discovery and development, eventually leading to translational research through the congregation of interdisciplinary groups of scientists and thus, contributing to our institutional biomedical and translational research vision and mission. Therefore, the specific aims of the MBTE core facility are to:
Specific Aim #1 : Continue to provide resource, instrumentation, and technical and hands-on support services for mammalian cell culture, tissue explant culture and bacterial culture.
Specific Aim #2 : Continue to offer tools, training and technical support services for different molecular technologies including conventional and real-time PCR, low density DNA array analysis, RNA interference, microRNA, promoter-reporter analysis, chromatin immunoprecipitation (ChIP) analysis, in-cell western analysis, epigenetic modifications and analysis of signaling pathways.
Specific Aim #3 : Continue to create a stimulating and supportive scientific atmosphere for the researchers at TSU through research development activities such as seminar programs, journal club meetings, and research presentations.
Specific Aim #4 : Continue to increase collaborative research efforts, and pursue exchange of technical capabilities with investigators at TSU and other research-intensive universities;and by attending technology workshops.
|Liang, Su; Bian, Xiaomei; Liang, Dong et al. (2016) Solution formulation development and efficacy of MJC13 in a preclinical model of castration-resistant prostate cancer. Pharm Dev Technol 21:121-6|
|Oyagbemi, Ademola Adetokunbo; Omobowale, Temidayo Olutayo; Adedapo, Adeolu Alex et al. (2016) Kolaviron, Biflavonoid Complex from the Seed of Garcinia kola Attenuated Angiotensin II- and Lypopolysaccharide-induced Vascular Smooth Muscle Cell Proliferation and Nitric Oxide Production. Pharmacognosy Res 8:S50-5|
|Robinson, Keila; Mock, Charlotta; Liang, Dong (2015) Pre-formulation studies of resveratrol. Drug Dev Ind Pharm 41:1464-9|
|Wang, Yan; Ye, Yuanqing; Lin, Jie et al. (2015) Genetic variants in matrix metalloproteinase genes as disposition factors for ovarian cancer risk, survival, and clinical outcome. Mol Carcinog 54:430-9|
|Liang, Su; Sanchez-Espiridion, Beatriz; Xie, Huan et al. (2015) Determination of proline in human serum by a robust LC-MS/MS method: application to identification of human metabolites as candidate biomarkers for esophageal cancer early detection and risk stratification. Biomed Chromatogr 29:570-7|
|Jimenez, Angelica; Adisa, Afolabi; Woodham, Cara et al. (2014) Determination of polycyclic aromatic hydrocarbons in roasted coffee. J Environ Sci Health B 49:828-35|
|Bell, Edward C; John, Mathew; Hughes, Rodney J et al. (2014) Ultra-performance liquid chromatographic determination of tocopherols and retinol in human plasma. J Chromatogr Sci 52:1065-70|
|Meng, Qing H; Xu, Enping; Hildebrandt, Michelle A T et al. (2014) Genetic variants in the fibroblast growth factor pathway as potential markers of ovarian cancer risk, therapeutic response, and clinical outcome. Clin Chem 60:222-32|
|Liang, Su; Bian, Xiaomei; Sivils, Jeffrey et al. (2014) Quantification of a New Anti-Cancer Molecule MJC13 Using a Rapid, Sensitive, and Reliable Liquid Chromatography-Tandem Mass Spectrometry Method. Am J Mod Chromatogr 1:1-11|
|Nguyen, Thao; Hlangothi, Duma; Martinez 3rd, Raul A et al. (2013) Charcoal burning as a source of polyaromatic hydrocarbons in waterpipe smoking. J Environ Sci Health B 48:1097-102|
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