Candidate: The K02 candidate, LaDora V. Thompson, is a cellular physiologist whose research is focused on molecular mechanisms underlying age-induced skeletal muscle deterioration. Although trained in muscle physiology, Dr. Thompson has acquired experience in muscle biochemistry and molecular biophysics. Her immediate goal is to focus intensively on her funded NIA R01 grant "Structural and Calcium Regulatory Proteins in Sarcopenia". Her long term goals are to maintain or exceed the current level of productivity in the following areas: 1) successfully attain continued extramurally-funded research focused on identifying underlying molecular mechanisms responsible for age-related muscle dysfunction, 2) continue to contribute to aging research by utilizing state of the art biophysical and )roteomic technology, 3) mentor students and post-doctoral fellows in aging research. The K02 Independent Scientist Award will provide the necessary funding for the candidate to reach these career goals by providing salary support to decrease her significant teaching load and allow her to spend the majority of her time on research. Environment: The environment for Dr. Thompson's continued research progress is outstanding. Her research collaborators, Drs. Thomas and Ferrington, are internationally known scientists in the fields of muscle biophysics and proteins modifications (proteomics). Drs. Thompson, Thomas and Ferrington are involved with the funded Center on Aging and the Center for Muscle and Muscle Disorders at the University of Minnesota. They have a history of successful collaborations and publications. Their combined expertise provides a novel approach to answering specific questions about age-related deterioration of muscle. Research: The overall aim of this research is to determine the molecular mechanisms underlying diminished performance of muscle with age (Aim 1). We are using electron paramagnetic resonance spectroscopy (Aim 2) and proteomic (Aim 3) techniques to study the effects of age on the structure, function, and post-translational modifications of myosin, the major contractile protein in muscle.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Scientist Development Award - Research (K02)
Project #
5K02AG021626-05
Application #
7176158
Study Section
National Institute on Aging Initial Review Group (NIA)
Program Officer
Williams, John
Project Start
2003-02-01
Project End
2008-01-31
Budget Start
2007-03-01
Budget End
2008-01-31
Support Year
5
Fiscal Year
2007
Total Cost
$108,135
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Physical Medicine & Rehab
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Kim, Jong-Hee; Thompson, LaDora V (2012) Differential effects of mild therapeutic exercise during a period of inactivity on power generation in soleus type I single fibers with age. J Appl Physiol (1985) 112:1752-61
Xu, Xin; Chen, Chiao-nan Joyce; Arriaga, Edgar A et al. (2010) Asymmetric superoxide release inside and outside the mitochondria in skeletal muscle under conditions of aging and disuse. J Appl Physiol (1985) 109:1133-9
Chen, Chiao-Nan Joyce; Brown-Borg, Holly M; Rakoczy, Sharlene G et al. (2010) Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress. J Gerontol A Biol Sci Med Sci 65:129-37
Prochniewicz, Ewa; Lowe, Dawn A; Spakowicz, Daniel J et al. (2008) Functional, structural, and chemical changes in myosin associated with hydrogen peroxide treatment of skeletal muscle fibers. Am J Physiol Cell Physiol 294:C613-26
Arora, P; Husom, A D; Ferrington, D A et al. (2008) Age-dependent effects of treadmill exercise during a period of inactivity. Exp Gerontol 43:668-73
Feng, Juan; Xie, Hongwei; Meany, Danni L et al. (2008) Quantitative proteomic profiling of muscle type-dependent and age-dependent protein carbonylation in rat skeletal muscle mitochondria. J Gerontol A Biol Sci Med Sci 63:1137-52
Chen, Chiao-nan; Ferrington, Deborah A; Thompson, LaDora V (2008) Carbonic anhydrase III and four-and-a-half LIM protein 1 are preferentially oxidized with muscle unloading. J Appl Physiol 105:1554-61
Chen, Chiao-nan Joyce; Brown-Borg, Holly M; Rakoczy, Sharlene G et al. (2008) Muscle disuse: adaptation of antioxidant systems is age dependent. J Gerontol A Biol Sci Med Sci 63:461-6
Prochniewicz, Ewa; Thompson, LaDora V; Thomas, David D (2007) Age-related decline in actomyosin structure and function. Exp Gerontol 42:931-8
Snow, LeAnn M; Fugere, Nicole A; Thompson, LaDora V (2007) Advanced glycation end-product accumulation and associated protein modification in type II skeletal muscle with aging. J Gerontol A Biol Sci Med Sci 62:1204-10

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