This is a renewal application for my K02 Independent Scientist Award that will enable me to continuously obtain maximum protected time at Seton Hall University (SHU) for working on my NIDA funded projects and mentoring research students and junior scientists. During the current K02 funding period, I have worked on and successfully renewed my long-standing R01 grant entitled, """"""""Morphine Actions on the Immune System"""""""", and have expanded my research projects as evidenced by receiving an R21 grant, """"""""Opiate's Effects on HIV- 1 Induced Inflammation in the CMS"""""""", and an R03 grant, """"""""Effects of Morphine on HIV-1 Neuroinvasion and Brain Changes in Mice"""""""" (Co-Pi with Dr. David J. Volsky), to study the interactions between drug abuse and HIV infection. I have implemented a confocal facility at SHU via my NSF Major Research Instrumentation grant, and have gained extensive experience in real time RT-PCR, fluorescence-based biological assays, and microarray technology. I have mentored research students and junior researchers, established an ongoing training program in the responsible conduct of research (RCR), and created a semester-wide Molecular Bioscience Meeting to enable graduate and undergraduate students to interact scientifically. In addition, I have helped establish the Institute of Neurolmmune Pharmacology (I-NIP), one of its missions is to translate bench work to the community. With this K02 grant renewal, I will: 1) Work on my R01 DA007058-17-22 (PI), and extend the studies proposed in my R21 DA019836-01-02 (PI) and R03 DA022169-01-02 (Co-Pi) awards to develop a new R01 project, respectively;2) Gain experience in using quantitative fluorescence microscopy, stereological procedures, and MicroBrightfield NeuroLeucida system in order to bring my in vivo research to the level of a single cell;3) Gain experience in animal behavioral testing to determine the effects of drug abuse in the presence of HIV infection;4) Bring basic science to the community by collaborating with clinical scientists and fulfilling the missions of the I-NIP;and 5) Continue to mentor research students, junior scientists, and under-represented students, emphasizing the RCR. My research projects and my initiatives in research-oriented training that are housed in the I-NIP are valuable assets to SHU, and the career development and research plans as proposed in this K02 renewal application have received tremendous support from the College of Arts and Sciences at Seton Hall University.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Scientist Development Award - Research (K02)
Project #
5K02DA016149-09
Application #
8033231
Study Section
Human Development Research Subcommittee (NIDA)
Program Officer
Purohit, Vishnudutt
Project Start
2003-02-05
Project End
2013-01-31
Budget Start
2011-02-01
Budget End
2012-01-31
Support Year
9
Fiscal Year
2011
Total Cost
$120,196
Indirect Cost
Name
Seton Hall University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
079324315
City
South Orange
State
NJ
Country
United States
Zip Code
07079
Song, Guohua; Nesil, Tanseli; Cao, Junran et al. (2016) Nicotine mediates expression of genes related to antioxidant capacity and oxidative stress response in HIV-1 transgenic rat brain. J Neurovirol 22:114-24
Yang, Zhongli; Nesil, Tanseli; Connaghan, Kaitlyn P et al. (2016) Modulation Effect of HIV-1 Viral Proteins and Nicotine on Expression of the Immune-Related Genes in Brain of the HIV-1 Transgenic Rats. J Neuroimmune Pharmacol 11:562-71
Nesil, Tanseli; Cao, Junran; Yang, Zhongli et al. (2015) Nicotine attenuates the effect of HIV-1 proteins on the neural circuits of working and contextual memories. Mol Brain 8:43
Vigorito, Michael; Connaghan, Kaitlyn P; Chang, Sulie L (2015) The HIV-1 transgenic rat model of neuroHIV. Brain Behav Immun 48:336-49
Morris, Niya L; Ippolito, Jill A; Curtis, Brenda J et al. (2015) Alcohol and inflammatory responses: summary of the 2013 Alcohol and Immunology Research Interest Group (AIRIG) meeting. Alcohol 49:1-6
Chen, Michael M; Zahs, Anita; Chang, Sulie L et al. (2014) Street smarts of science for students. Nat Immunol 15:997-9
Wiedinger, Kari; Bitsaktsis, Constantine; Chang, Sulie (2014) Reactivation of human polyomaviruses in immunocompromised states. J Neurovirol 20:1-8
Abbondanzo, Susan J; Chang, Sulie L (2014) HIV-1 transgenic rats display alterations in immunophenotype and cellular responses associated with aging. PLoS One 9:e105256
Cao, Junran; Wang, Shaolin; Wang, Ju et al. (2013) RNA deep sequencing analysis reveals that nicotine restores impaired gene expression by viral proteins in the brains of HIV-1 transgenic rats. PLoS One 8:e68517
Sarkar, Sraboni; Chang, Sulie L (2013) Ethanol concentration-dependent alterations in gene expression during acute binge drinking in the HIV-1 transgenic rat. Alcohol Clin Exp Res 37:1082-90

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