This Independent Scientist Award (K02) application is based on the applicant's recently funded NIH R01 award from NIDCR (DE 017205) for the years of 2006-2010. The parental R01 award entitled """"""""Role of TFII-I in Craniofacial Development"""""""" addresses the function of the TFII-I transcription factor in orofacial development. The proposed research presented in the """"""""Research Plan"""""""" section of this K02 application does not have significant changes compared with the studies proposed in the original R01 application, except that some progress made since then is incorporated in the preliminary data section of this application. The research approaches and experimental design proposed in the original R01 application are largely based on the applicant's scientific training and expertise in mouse genetics and molecular biology. The applicant fully realizes the importance of multi-discipline in conducting competitive and high quality research, which is essential for a successful scientific career. Therefore, the applicant plans to acquire additional expertise and learn new systems to promote the original proposal to a higher level and extent the principal investigator's scientific expertise in functional screening, protein biochemistry and proteomics, and systems biology. The details and rationale are described in the following section of the candidate's """"""""career developmental plan"""""""".

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Scientist Development Award - Research (K02)
Project #
5K02DE018412-05
Application #
8089405
Study Section
NIDCR Special Grants Review Committee (DSR)
Program Officer
King, Lynn M
Project Start
2007-07-01
Project End
2012-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
5
Fiscal Year
2011
Total Cost
$101,075
Indirect Cost
Name
University of Connecticut
Department
Dentistry
Type
Schools of Dentistry
DUNS #
022254226
City
Farmington
State
CT
Country
United States
Zip Code
06030
Makeyev, Aleksandr V; Enkhmandakh, Badam; Hong, Seung-Hyun et al. (2012) Diversity and complexity in chromatin recognition by TFII-I transcription factors in pluripotent embryonic stem cells and embryonic tissues. PLoS One 7:e44443
Bayarsaihan, Dashzeveg; Makeyev, Aleksandr V; Enkhmandakh, Badam (2012) Epigenetic modulation by TFII-I during embryonic stem cell differentiation. J Cell Biochem 113:3056-60
Chimge, Nyam-Osor; Makeyev, Aleksandr V; Waigel, Sabine J et al. (2012) PI3K/Akt-dependent functions of TFII-I transcription factors in mouse embryonic stem cells. J Cell Biochem 113:1122-31
Bayarsaihan, D (2011) Epigenetic mechanisms in inflammation. J Dent Res 90:9-17
Makeyev, Aleksandr V; Bayarsaihan, Dashzeveg (2011) Molecular basis of Williams-Beuren syndrome: TFII-I regulated targets involved in craniofacial development. Cleft Palate Craniofac J 48:109-16
Chimge, Nyam-Osor; Bayarsaihan, Dashzeveg (2010) Generation of neural crest progenitors from human embryonic stem cells. J Exp Zool B Mol Dev Evol 314:95-103
Enkhmandakh, Badam; Makeyev, Aleksandr V; Erdenechimeg, Lkhamsuren et al. (2009) Essential functions of the Williams-Beuren syndrome-associated TFII-I genes in embryonic development. Proc Natl Acad Sci U S A 106:181-6
Makeyev, Aleksandr V; Bayarsaihan, Dashzeveg (2009) Alternative splicing and promoter use in TFII-I genes. Gene 433:16-25
Makeyev, Aleksandr V; Bayarsaihan, Dashzeveg (2009) New TFII-I family target genes involved in embryonic development. Biochem Biophys Res Commun 386:554-8
Chimge, Nyam-Osor; Makeyev, Aleksandr V; Ruddle, Frank H et al. (2008) Identification of the TFII-I family target genes in the vertebrate genome. Proc Natl Acad Sci U S A 105:9006-10