The applicants laboratory has recently identified five trans-acting regulatory factors governing prokaryotic gene expression in response to changes in oxygen tension and light intensity. During the proposed funding period, each of these regulatory factors will be genetically and biochemically characterized for their role in controlling gene expression in response to oxygen and light. Two """"""""oxygen responding"""""""" regulatory proteins are additional members of a family of two-component response regulators which indicates that one regulatory protein is a membrane spanning sensor kinase that transmits information about anaerobioses via phosphorylation of a DNA binding response regulator. An additional sensor kinase is described which is thought to be involved in sensing light intensity by monitoring the redox state of the electron transport chain. This additional redox responding regulator is structurally similar to a protein in mitochondria which is known to be involved in expression of cytochrome oxidase; a protein implicated in degenerative diseases such as Parkinson's and Ischemic heart disease. The laboratory is also characterizing a light sensing regulatory protein involved in controlling expression of the enzyme S-adenosyl homocysteine hydrolase (SAHase). SAHase is an important contributing factor in the cytotoxic effects of adenosine leading to severe immune deficiency in patents with adenosine deaminase (ADA) deficiency.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Modified Research Career Development Award (K04)
Project #
1K04GM000618-01
Application #
3073017
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1993-07-01
Project End
1998-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Indiana University Bloomington
Department
Type
Schools of Arts and Sciences
DUNS #
006046700
City
Bloomington
State
IN
Country
United States
Zip Code
47401
Bird, T H; Du, S; Bauer, C E (1999) Autophosphorylation, phosphotransfer, and DNA-binding properties of the RegB/RegA two-component regulatory system in Rhodobacter capsulatus. J Biol Chem 274:16343-8
Du, S; Bird, T H; Bauer, C E (1998) DNA binding characteristics of RegA. A constitutively active anaerobic activator of photosynthesis gene expression in Rhodobacter capsulatus. J Biol Chem 273:18509-13
Ponnampalam, S N; Elsen, S; Bauer, C E (1998) Aerobic repression of the Rhodobacter capsulatus bchC promoter involves cooperative interactions between CrtJ bound to neighboring palindromes. J Biol Chem 273:30757-61
Nickens, D G; Bauer, C E (1998) Analysis of the puc operon promoter from Rhodobacter capsulatus. J Bacteriol 180:4270-7
Xiong, J; Inoue, K; Bauer, C E (1998) Tracking molecular evolution of photosynthesis by characterization of a major photosynthesis gene cluster from Heliobacillus mobilis. Proc Natl Acad Sci U S A 95:14851-6
Elsen, S; Ponnampalam, S N; Bauer, C E (1998) CrtJ bound to distant binding sites interacts cooperatively to aerobically repress photopigment biosynthesis and light harvesting II gene expression in Rhodobacter capsulatus. J Biol Chem 273:30762-9
Ponnampalam, S N; Bauer, C E (1997) DNA binding characteristics of CrtJ. A redox-responding repressor of bacteriochlorophyll, carotenoid, and light harvesting-II gene expression in Rhodobacter capsulatus. J Biol Chem 272:18391-6
Suzuki, J Y; Bollivar, D W; Bauer, C E (1997) Genetic analysis of chlorophyll biosynthesis. Annu Rev Genet 31:61-89
Oster, U; Bauer, C E; Rudiger, W (1997) Characterization of chlorophyll a and bacteriochlorophyll a synthases by heterologous expression in Escherichia coli. J Biol Chem 272:9671-6
Smith, C A; Suzuki, J Y; Bauer, C E (1996) Cloning and characterization of the chlorophyll biosynthesis gene chlM from Synechocystis PCC 6803 by complementation of a bacteriochlorophyll biosynthesis mutant of Rhodobacter capsulatus. Plant Mol Biol 30:1307-14

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