Abstract

application abstract): This KO8 award is requested to permit Dr. Givens to acquire the skills to be a biomedical researcher. Dr. Givens is doing his residency training in veterinary medicine and has enrolled in a Ph.D. program. In vitro production of embryos has become a useful therapy for increasing reproductive performance of animals. However, in vitro produced bovine embryos are potential vectors for transmission of the ruminant pestivirus known as bovine viral diarrhea virus (BVDV). The hypothesis of the proposed research is that N-butyl-deoxynojirimycin (NB-DNJ) may be used in the culture medium of developing bovine embryos during the first 7 days of development to limit or prevent the transmission of BVDV while not inhibiting the development of the embryo into a normal calf. The use of a broad spectrum antiviral agent in an in vitro embryo production system may inhibit transmission of viruses associated with in vitro fertilized embryos. The objectives are to (1) evaluate viral strain affinity for association with IVF embryos and potential for embryo-associated viral transmission, (2) determine the BVDV inhibitory concentration for bovine conceptus development of NB-DNJ, (3) evaluate the ability of NB-DNJ to inhibit BVDV replication and association with IVF embryos in a contaminated embryo culture system, and (4) evaluate the ability of IVF embryos exposed to NB-DNJ to initiate pregnancy and develop to normal offspring.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08AI001728-03
Application #
6532622
Study Section
Microbiology and Infectious Diseases B Subcommittee (MID)
Program Officer
Meegan, James M
Project Start
2000-09-30
Project End
2003-12-31
Budget Start
2002-09-01
Budget End
2003-12-31
Support Year
3
Fiscal Year
2002
Total Cost
$119,894
Indirect Cost

  Institution

Name
Auburn University at Auburn
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
City
Auburn University
State
AL
Country
United States
Zip Code
36849

  Publications

Givens, M D; Stringfellow, D A; Riddell, K P et al. (2006) Normal calves produced after transfer of in vitro fertilized embryos cultured with an antiviral compound. Theriogenology 65:344-55
Givens, M D; Galik, P K; Riddell, K P et al. (2005) Effects of aromatic cationic molecules on bovine viral diarrhea virus and embryonic development. Theriogenology 63:1984-94
Givens, M Daniel; Waldrop, Julie G (2004) Bovine viral diarrhea virus in embryo and semen production systems. Vet Clin North Am Food Anim Pract 20:21-38
Givens, M Daniel; Heath, Allen M; Brock, Kenny V et al. (2003) Detection of bovine viral diarrhea virus in semen obtained after inoculation of seronegative postpubertal bulls. Am J Vet Res 64:428-34
Givens, M Daniel; Dykstra, Christine C; Brock, Kenny V et al. (2003) Detection of inhibition of bovine viral diarrhea virus by aromatic cationic molecules. Antimicrob Agents Chemother 47:2223-30