Studies to improve the effectiveness and efficiency of platelet transfusion therapy are proposed with emphasis on transfusion of single-donor concentrates to alloimmunized patients. A comparative study of various methods for detection of platelet antibodies by indirect testing utilizing different methodologies will be undertaken, and these will be evaluated for sensitivity, specificity and feasibility of being applied on a large scale. The applicant will also undertake the analysis of information on 2,500 single-donor platelet transfusions performed previously at The Blood Center of Southeastern Wisconsin in an attempt to gain new insights into requirements for donor-recipient antigen compatibility and other causes of the variable responses observed in many patients. In subsequent studies, the importance of circulating immune complexes in affecting the response of patients to single-donor and random-donor platelets will be investigated, and a prospective study to determine whether reduction of the leukocyte content of platelet concentrates reduces or delays alloimunization will be implemented. Finally, platelet utilization patterns in southeastern Wisconsin will be studied and strategies to improve platelet transfusion practices will be tested on a controlled basis.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL001376-05
Application #
3081806
Study Section
(SRC)
Project Start
1984-04-01
Project End
1989-03-31
Budget Start
1988-04-01
Budget End
1989-03-31
Support Year
5
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Bloodcenter of Wisconsin, Inc.
Department
Type
DUNS #
City
Milwaukee
State
WI
Country
United States
Zip Code
53233
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Bussel, J B; Berkowitz, R L; McFarland, J G et al. (1988) Antenatal treatment of neonatal alloimmune thrombocytopenia. N Engl J Med 319:1374-8
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