The activation of Factor X by Factor IXa in the presence of Factor VIIICa, phospholipid (or platelets) and calcium will be studied in a procine system. Recently developed methods for purifying porcine Factor VIIIC to apparent homogeneity make a rigorous biochemical analysis of this system possible for the first time. An experimental approach is outlined to explore the equilibrium interactions of the various components of the system and the kinetics of the overall reaction. The experimental approach relies heavily upon past experience with the prothrombinase system which may be very similar to intrinsic pathway Factor X activation. This study will contribute to the understanding of the biology of Factor VIII, the Factor X activation in general, and will provide necessary data for the analysis of the possible interaction of the intrinsic pathway X activator complex and the prothrombinase complex.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL001538-05
Application #
3081904
Study Section
(SRC)
Project Start
1984-07-01
Project End
1987-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
5
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Vermont & St Agric College
Department
Type
Schools of Medicine
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
Lollar, P; Parker, C G; Tracy, R P (1988) Molecular characterization of commercial porcine factor VIII concentrate. Blood 71:137-43
Lollar, P (1987) Heterogeneous, ideal associations at sedimentation equilibrium. Biophys Chem 28:245-51
Lollar, P; Parker, C G (1987) Stoichiometry of the porcine factor VIII-von Willebrand factor association. J Biol Chem 262:17572-6
Lollar, P; Parker, C G; Kajenski, P J et al. (1987) Degradation of coagulation proteins by an enzyme from Malayan pit viper (Akistrodon rhodostoma) venom. Biochemistry 26:7627-36