Abstract

Osteoclast More than twenty years ago Walker proved that bone resorbing cells called """"""""Osteoclasts"""""""" came from hematopoetic precursors, Today other investigators have shown that osteoclasts were influences by systemic hormones (parathyroid hormone, 1, 25-dihydroxyvitamin D3, calcitonin), cytokines (IL-1, IL-3, IL-6,) and hematopoetic factors/colony growth factors. Yet most of these stromal or marrow cultures involved in vitro systems. My project uses an in vivo system that has been proven by Minkin (1991) to develop osteoclasts from their precursors in a chemically defined medium. I will characterize a gene related to osteoclastogenesis via a method called mRNA differential display developed by Pardee and Liang at Harvard Medical School. Metatarsals from embryonic mice were harvested at stages before and after osteoclast presentation, RNA extracted, and cDNA probes made via PCR. Currently, hands are being sequenced.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
3K16DE000275-05S1
Application #
3732451
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

McDermott, Nancy E; Chuang, Sung-Kiang; Woo, Valerie V et al. (2006) Maxillary sinus augmentation as a risk factor for implant failure. Int J Oral Maxillofac Implants 21:366-74
Chuang, S K; Cai, T; Douglass, C W et al. (2005) Frailty approach for the analysis of clustered failure time observations in dental research. J Dent Res 84:54-8
Chuang, S-K; Hatch, J P; Rugh, J et al. (2005) Multi-center randomized clinical trials in oral and maxillofacial surgery: modeling of fixed and random effects. Int J Oral Maxillofac Surg 34:341-4
Treister, Nathaniel S; Woo, Sook-Bin; O'Holleran, Eileen W et al. (2005) Oral chronic graft-versus-host disease in pediatric patients after hematopoietic stem cell transplantation. Biol Blood Marrow Transplant 11:721-31
Woo, Valerie V; Chuang, Sung-Kiang; Daher, Shadi et al. (2004) Dentoalveolar reconstructive procedures as a risk factor for implant failure. J Oral Maxillofac Surg 62:773-80
Halpern, Leslie R; Carter, Jeffrey B; Chuang, Sung-Kiang et al. (2003) A comparison of 2 consultation and treatment strategies to manage impacted third molars. J Oral Maxillofac Surg 61:779-84
Basile, John R; Eichten, Alexandra; Zacny, Valerie et al. (2003) NF-kappaB-mediated induction of p21(Cip1/Waf1) by tumor necrosis factor alpha induces growth arrest and cytoprotection in normal human keratinocytes. Mol Cancer Res 1:262-70
McDermott, Nancy E; Chuang, Sung-Kiang; Woo, Valerie V et al. (2003) Complications of dental implants: identification, frequency, and associated risk factors. Int J Oral Maxillofac Implants 18:848-55
Chuang, S K; Tian, L; Wei, L J et al. (2002) Predicting dental implant survival by use of the marginal approach of the semi-parametric survival methods for clustered observations. J Dent Res 81:851-5
Chuang, S K; Wei, L J; Douglass, C W et al. (2002) Risk factors for dental implant failure: a strategy for the analysis of clustered failure-time observations. J Dent Res 81:572-7

Showing the most recent 10 out of 32 publications