This K22 application is to support the scholarly training and career development of Dr. Xiaofeng Charles Zhou to become an independent investigator in head and neck cancer research. This application details the strengths and uniqueness of this proposal pertaining to the candidate, the mentor, the career development plan, the environment and the research plan. The proposed research is to test the hypothesis that there are genomic determinants that distinguish progressing from non-progressing oral precancers. The overall risk of malignant transformation of all grades of oral precancers is approximately 18%. It is impossible to predict progressiveness of any one lesion based on conventional clinical and histological features. Genomic-based new classifications are essential to distinguish the progressing from non-progressing oral precancers. The proposed research aims to determine genome-wide differential allelic imbalance (LOH) profiles for progressing and non-progressing oral precancers.
Specific Aim 1 is to establish and optimize a series of innovative emerging genomic tools, to be used consecutively for the application on oral precancers in vivo. This will be accomplished during the Scholar Development Phase (2 years).
Specific Aim 2, to be performed in the Faculty Transition Phase, is to determine the genome wide LOH profiles in progressing and non-progressing oral precancers and generate molecular classification for the prediction of oral precancer progression.
Specific Aim 3 is to identify candidate genes for oral precancer progression in the identified LOH regions. Functional and biological tests are in place to evaluate the functional contribution of candidate genes to the tumor progression process. A career development plan is in place to prepare the candidate for translational head and neck cancer research. Defined and evaluative milestones are in place to assess the scientific progress of the candidate, particularly during transition to the Faculty Transition Phase. In addition to the research training, the candidate will receive didactic training in bioethics and responsible conduct of research, oral pathology, biostatistics, and grant writing. The outcome of this award will be a well prepared and competitive young scientist committed to a career in translational head and neck cancer research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Career Transition Award (K22)
Project #
3K22DE014847-05S1
Application #
7840924
Study Section
Special Emphasis Panel (ZDE1-LK (17))
Program Officer
Hardwick, Kevin S
Project Start
2009-06-02
Project End
2010-05-31
Budget Start
2009-06-02
Budget End
2010-05-31
Support Year
5
Fiscal Year
2009
Total Cost
$9,288
Indirect Cost
Name
University of Illinois at Chicago
Department
Dentistry
Type
Schools of Dentistry
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612
Jin, Yi; Chen, Zujian; Liu, Xiqiang et al. (2013) Evaluating the microRNA targeting sites by luciferase reporter gene assay. Methods Mol Biol 936:117-27
Wang, Cheng; Liu, Xiqiang; Chen, Zujian et al. (2013) Polycomb group protein EZH2-mediated E-cadherin repression promotes metastasis of oral tongue squamous cell carcinoma. Mol Carcinog 52:229-36
Wang, Cheng; Liu, Xiqiang; Huang, Hongzhang et al. (2012) Deregulation of Snai2 is associated with metastasis and poor prognosis in tongue squamous cell carcinoma. Int J Cancer 130:2249-58
Chen, Zujian; Jin, Yi; Yu, Dongsheng et al. (2012) Down-regulation of the microRNA-99 family members in head and neck squamous cell carcinoma. Oral Oncol 48:686-91
Jin, Yi; Wang, Cheng; Liu, Xiqiang et al. (2011) Molecular characterization of the microRNA-138-Fos-like antigen 1 (FOSL1) regulatory module in squamous cell carcinoma. J Biol Chem 286:40104-9
Wang, Cheng; Huang, Hongzhang; Huang, Zhiquan et al. (2011) Tumor budding correlates with poor prognosis and epithelial-mesenchymal transition in tongue squamous cell carcinoma. J Oral Pathol Med 40:545-51
Sharma, Sanjai; Liao, Wei; Zhou, Xiaofeng et al. (2011) Exon 11 skipping of E-cadherin RNA downregulates its expression in head and neck cancer cells. Mol Cancer Ther 10:1751-9
Jiang, Lu; Dai, Yang; Liu, Xiqiang et al. (2011) Identification and experimental validation of G protein alpha inhibiting activity polypeptide 2 (GNAI2) as a microRNA-138 target in tongue squamous cell carcinoma. Hum Genet 129:189-97
Chen, Zujian; Liu, Xiqiang; Wang, Cheng et al. (2011) p53 regulates the expression of human angiotensin II AT(2) receptor interacting protein (ATIP1) gene. Oncol Lett 2:919-922
Liu, Xiqiang; Wang, Cheng; Chen, Zujian et al. (2011) MicroRNA-138 suppresses epithelial-mesenchymal transition in squamous cell carcinoma cell lines. Biochem J 440:23-31

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