Dr. Angela Peck is a 3rd year fellow in Pediatric Infectious Diseases at the University of Washington. Her long-term goal is an academic career, with a clinical research program that uses novel laboratory assaysto study the epidemiology and pathogenesis of respiratory virus infections among healthy and immuno- compromised patients. She has long-standing interests and research experience in the field of respiratory viruses, and her proposed career development plan incorporates an intense, closely mentored patient- oriented training environment with a plan for a comprehensive didactic curriculum. Dr. Peck will investigate parainfluenza virus (PIV) infections after hematopoietic cell transplant (HCT). PIV infections are not only common, but often severe and fatal in HCT recipients, and frequently cause pulmonary sequelae among long-term survivors. Even upper respiratory infection with PIV is associated with increased mortality after HCT. Preliminary data suggest that PIV infection occurs frequently in the absence of symptomsamong HCT recipients. The overall hypothesis of this proposal is that asymptomatic, or subclinical, PIV shedding in HCT recipients is common, related to host immunologic factors, and may increase PIV transmission to others.
For Aims 1 and 2, a cohort of 500 HCT recipients will be followed with nasal wash sampling and serum collection for 1 year after HCT. By testing the respiratory samples for PIV using RT-PCR, these studies will define frequency and duration of PIV shedding, risk factors for symptomatic progression, and correlation of PIV virus quantitation and disease severity. Serum samples from patients in this cohort will be tested for PIV-specific neutralizing antibody, and a nested case-control analysiswill be performed to determine whether humoral immunity is protective against acquisition and progression of PIV infection in HCT recipients.
Aim 3 is to develop and validate a new self-collection method of nasal secretions for detection of respiratory viruses by RT-PCR. This method will be compared to nasal washes, the current standard of care. By characterizing PIV infections that occur without symptoms after HCT, and studying factors which affect whether patients acquire PIV and progress to serious disease, these studies may ultimately provide targets for PIV prevention and therapy. Development of a self-collection method will allow for frequent sampling in outpatients, and provide a foundation for future studies of PIV pathogenesis and transmission.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Mentored Patient-Oriented Research Career Development Award (K23)
Project #
5K23HL091059-05
Application #
8197708
Study Section
Special Emphasis Panel (ZHL1-CSR-R (O1))
Program Officer
Mondoro, Traci
Project Start
2008-02-06
Project End
2013-03-30
Budget Start
2011-12-01
Budget End
2013-03-30
Support Year
5
Fiscal Year
2012
Total Cost
$136,879
Indirect Cost
$10,139
Name
Seattle Children's Hospital
Department
Type
DUNS #
048682157
City
Seattle
State
WA
Country
United States
Zip Code
98105
Chu, Helen Y; Englund, Janet A; Podczervinski, Sara et al. (2014) Nosocomial transmission of respiratory syncytial virus in an outpatient cancer center. Biol Blood Marrow Transplant 20:844-51
Seo, Sachiko; Xie, Hu; Campbell, Angela P et al. (2014) Parainfluenza virus lower respiratory tract disease after hematopoietic cell transplant: viral detection in the lung predicts outcome. Clin Infect Dis 58:1357-68
Seo, S; Xie, H; Karron, R A et al. (2014) Parainfluenza virus type 3 Ab in allogeneic hematopoietic cell transplant recipients: factors influencing post-transplant Ab titers and associated outcomes. Bone Marrow Transplant 49:1205-11
Seo, Sachiko; Campbell, Angela P; Xie, Hu et al. (2013) Outcome of respiratory syncytial virus lower respiratory tract disease in hematopoietic cell transplant recipients receiving aerosolized ribavirin: significance of stem cell source and oxygen requirement. Biol Blood Marrow Transplant 19:589-96
Campbell, Angela P; Kuypers, Jane; Englund, Janet A et al. (2013) Self-collection of foam nasal swabs for respiratory virus detection by PCR among immunocompetent subjects and hematopoietic cell transplant recipients. J Clin Microbiol 51:324-7
Renaud, Christian; Campbell, Angela P (2011) Changing epidemiology of respiratory viral infections in hematopoietic cell transplant recipients and solid organ transplant recipients. Curr Opin Infect Dis 24:333-43
Campbell, Angela P; Chien, Jason W; Kuypers, Jane et al. (2010) Respiratory virus pneumonia after hematopoietic cell transplantation (HCT): associations between viral load in bronchoalveolar lavage samples, viral RNA detection in serum samples, and clinical outcomes of HCT. J Infect Dis 201:1404-13
Milano, Filippo; Campbell, Angela P; Guthrie, Katherine A et al. (2010) Human rhinovirus and coronavirus detection among allogeneic hematopoietic stem cell transplantation recipients. Blood 115:2088-94
Campbell, Angela P; Jacob, Shevin T; Kuypers, Jane et al. (2010) Respiratory failure caused by 2009 novel influenza A/H1N1 in a hematopoietic stem-cell transplant recipient: Detection of extrapulmonary H1N1 RNA and use of intravenous peramivir. Ann Intern Med 152:619-20