Myeloproliferative disorders (MPDs) characterized by prominent myelofibrosis have a poor prognosis with no effective therapies outside of allogeneic stem cell transplantation. Recently, several groups identified a gain- of-function mutation in Janus Kinase 2 (JAK2 V617F) in the majority of patients with MPDs, suggesting that JAK2 is a potential therapeutic target in these diseases, including the myelofibrotic disorders. A primary in vitro kinase screen against purified JAK2 enzyme showed that the small-molecule multikinase inhibitor lestaurtinib (CEP-701) inhibits its activity with an IC50 of 1 nM. Based on these data, I found that CEP-701 selectively inhibits growth of primary cells from subjects with MPDs at doses that are clinically achievable. These studies led to the initiation of an MPD Research Consortium sponsored study, of which I am the study chair. The objectives of this proposal are first, to determine the safety and activity of CEP-701 in subjects with myelofibrosis;second, to use clinical samples obtained during this study to estimate optimal biologic dose and the relationship between inhibition of JAK2 mediated signaling and clinical response. Finally, to determine whether other previously identified disease modifiers are regulated by JAK2, I will test whether NF-E2 and Bcl-xL expression can be modified by JAK2 inhibition in primary patient cells. Together, these studies will provide a basis for predicting response and refining drug targets in the treatment of MPDs. This proposal outlines a 5 year plan for developing my independent career as a physician-scientist with an expertise in late preclinical testing and early phase studies of novel therapies for hematologic malignancies. It will be mentored by Dr. Martin Carroll, a leader both in the field of signal transduction in myeloid disorders and in related translational studies in experimental therapeutics. This award will allow me to complete a Master's Degree in Translational Research, and will provide the experience and formal training required to establish my career in translational medicine.

Public Health Relevance

Approximately 15,000 new cases of myeloproliferative disorders are diagnosed in the United States each year. This research project focuses on novel, targeted therapies for this group of diseases, and may reveal key aspects of the biology of the disease and general principles of targeted therapy.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Mentored Patient-Oriented Research Career Development Award (K23)
Project #
5K23HL093366-02
Application #
7813857
Study Section
Special Emphasis Panel (ZHL1-CSR-R (F1))
Program Officer
Mondoro, Traci
Project Start
2009-05-01
Project End
2013-03-31
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
2
Fiscal Year
2010
Total Cost
$137,295
Indirect Cost
Name
University of Pennsylvania
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Hexner, Elizabeth O; Luger, Selina M; Reshef, Ran et al. (2016) Infusion of CD3/CD28 costimulated umbilical cord blood T cells at the time of single umbilical cord blood transplantation may enhance engraftment. Am J Hematol 91:453-60
Gotlib, Jason; Kluin-Nelemans, Hanneke C; George, Tracy I et al. (2016) Efficacy and Safety of Midostaurin in Advanced Systemic Mastocytosis. N Engl J Med 374:2530-41
Hexner, Elizabeth O; Mascarenhas, John; Prchal, Josef et al. (2015) Phase I dose escalation study of lestaurtinib in patients with myelofibrosis. Leuk Lymphoma 56:2543-51
Babushok, Daria; Hexner, Elizabeth (2014) Allogeneic transplantation for myelofibrosis: for whom, when, and what are the true benefits? Curr Opin Hematol 21:114-22
Kalota, Anna; Jeschke, Grace R; Carroll, Martin et al. (2013) Intrinsic resistance to JAK2 inhibition in myelofibrosis. Clin Cancer Res 19:1729-39
Huang, Jian; Nguyen-McCarty, Michelle; Hexner, Elizabeth O et al. (2012) Maintenance of hematopoietic stem cells through regulation of Wnt and mTOR pathways. Nat Med 18:1778-85