I am a Clinician Investigator in the Division of Infectious Diseases at University of Virginia and am submitting this one-time K24 renewal application to further extend my molecular diagnostics research into patient oriented research (POR) and to provide protected time to mentor both my direct and institutional trainees. In the 15 years since completion of ID fellowship I have developed a research program in several domains of tropical infectious diseases, now largely focused on the development of molecular diagnostic tools for research.
Aim 1 : In the first term of this grant I established the University of Virginia School of Medicine K-award mentoring program, which entails a series of Symposia and meetings geared towards both those applying for and those who have already received NIH K or similar awards. This K24 grant will allow me to continue and extend this important program further. This grant will also allow protected time to directly mentor my trainees in POR, which currently consists of 3 K23 recipients and 1 Infectious Disease fellow, working in areas spanning MDR TB, pediatric TB, childhood diarrhea, and antimicrobial resistance, each with a diagnostic emphasis.
Aim 2 : I will also extend my personal research program into POR. Currently funded work includes the use of molecular diagnostics for both case- and population-level etiologic diagnosis of diarrhea and molecular diagnosis of MDR Tuberculosis. The most mature work involves diarrheal etiology, and our latest data strongly supports entering into clinical trials for Enterotoxigenic E. coli vaccines and new Cryptosporidium therapeutics. This K24 will allow me protected time to insert these molecular diagnostics into these trials as primary or secondary outcomes and gain needed skills in clinical trial design and data analysis. Finally, consonant with the K24 mechanism, I will expand my research new areas such as molecular diagnostics for antimicrobial resistance.

Public Health Relevance

This proposal will provide Dr. Houpt protected time for direct mentoring of Infectious Disease fellows and junior faculty in patient-oriented research, as well as for extending institutional mentoring programs across the entire UVA School of Medicine. This proposal will also expand our leading-edge molecular diagnostics development into patient oriented research, specifically clinical trials for Enterotoxigenic E. coli vaccines and Cryptosporidium therapeutics.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Midcareer Investigator Award in Patient-Oriented Research (K24)
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Microbiology and Infectious Diseases B Subcommittee (MID)
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Glock, Jonathan A
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University of Virginia
Internal Medicine/Medicine
Schools of Medicine
United States
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Pholwat, Suporn; Liu, Jie; Stroup, Suzanne et al. (2017) The Malaria TaqMan Array Card Includes 87 Assays for Plasmodium falciparum Drug Resistance, Identification of Species, and Genotyping in a Single Reaction. Antimicrob Agents Chemother 61:
Foongladda, S; Banu, S; Pholwat, S et al. (2016) Comparison of TaqMan(®) Array Card and MYCOTB(TM) with conventional phenotypic susceptibility testing in MDR-TB. Int J Tuberc Lung Dis 20:1105-12
Pholwat, Suporn; Sakai, Fuminori; Turner, Paul et al. (2016) Development of a TaqMan Array Card for Pneumococcal Serotyping on Isolates and Nasopharyngeal Samples. J Clin Microbiol 54:1842-1850
Liu, Jie; Ochieng, Caroline; Wiersma, Steve et al. (2016) Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus. J Clin Microbiol 54:49-58
Pholwat, Suporn; Stroup, Suzanne; Heysell, Scott et al. (2016) eis Promoter C14G and C15G Mutations Do Not Confer Kanamycin Resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother 60:7522-7523
Heysell, Scott K; Ahmed, Shahriar; Ferdous, Sara Sabrina et al. (2015) Quantitative drug-susceptibility in patients treated for multidrug-resistant tuberculosis in Bangladesh: implications for regimen choice. PLoS One 10:e0116795
Foongladda, Suporn; Klayut, Wiphat; Pholwat, Suporn et al. (2015) Comparison and development of pyrazinamide susceptibility testing methods for tuberculosis in Thailand. Diagn Microbiol Infect Dis 83:270-3
Pholwat, Suporn; Liu, Jie; Stroup, Suzanne et al. (2015) Integrated microfluidic card with TaqMan probes and high-resolution melt analysis to detect tuberculosis drug resistance mutations across 10 genes. MBio 6:e02273
Pholwat, Suporn; Stroup, Suzanne; Gratz, Jean et al. (2014) Pyrazinamide susceptibility testing of Mycobacterium tuberculosis by high resolution melt analysis. Tuberculosis (Edinb) 94:20-5
Foongladda, Suporn; Klayut, Wiphat; Chinli, Rattapha et al. (2014) Use of mycobacteriophage quantitative PCR on MGIT broths for a rapid tuberculosis antibiogram. J Clin Microbiol 52:1523-8

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