Core - Microinjection

Smith, James R.

Abstract

All the projects will make use of the microinjection facility to introduce macromolecules (primarily RNA and DNA) into cultured mammalian cells. This centralized facility will provide equipment, supplies, and personnel for the whole program. This is a very cost effective way for all of the investigators to have access to techniques requiring skilled personnel and specialized equipment. The major equipment needed for microinjection is available in Dr. J. Smith's laboratory and will be used in this core. Major items available include a Nikon Diaphot inverted phase-contrast microscope, DeFonbrune micromanipulator, a Brown-Flaming micropipette puller, and video camera and monitor.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute on Aging (NIA)
• Type: Research Program Projects (P01)
• Project #: 5P01AG007123-05
• Application #: 3790220
• Support Year: 5
• Fiscal Year: 1992

Institution

• Name: Baylor College of Medicine
• DUNS #: 074615394
• City: Houston
• State: TX
• Country: United States
• Zip Code: 77030

Publications

Duttaroy, A; Qian, J F; Smith, J S et al. (1997) Up-regulated P21CIP1 expression is part of the regulation quantitatively controlling serum deprivation-induced apoptosis. J Cell Biochem 64:434-46

Smith, J R; Nakanishi, M; Robetorye, R S et al. (1996) Studies demonstrating the complexity of regulation and action of the growth inhibitory gene SDI1. Exp Gerontol 31:327-35

Yang, L; Didenko, V V; Noda, A et al. (1995) Increased expression of p21Sdi1 in adrenocortical cells when they are placed in culture. Exp Cell Res 221:126-31

Kaul, S C; Wadhwa, R; Matsuda, Y et al. (1995) Mouse and human chromosomal assignments of mortalin, a novel member of the murine hsp70 family of proteins. FEBS Lett 361:269-72

Wadhwa, R; Pereira-Smith, O M; Reddel, R R et al. (1995) Correlation between complementation group for immortality and the cellular distribution of mortalin. Exp Cell Res 216:101-6

Nakanishi, M; Robetorye, R S; Pereira-Smith, O M et al. (1995) The C-terminal region of p21SDI1/WAF1/CIP1 is involved in proliferating cell nuclear antigen binding but does not appear to be required for growth inhibition. J Biol Chem 270:17060-3

Hensler, P J; Pereira-Smith, O M (1995) Human replicative senescence. A molecular study. Am J Pathol 147:1-8

Khaoustov, V I; Ozer, A; Smith, J R et al. (1995) Induction of senescent cell-derived inhibitor of DNA synthesis gene, SDI1, in hepatoblastoma (HepG2) cells arrested in the G2-phase of the cell cycle by 9-nitrocamptothecin. Lab Invest 73:118-27

Nakanishi, M; Robetorye, R S; Adami, G R et al. (1995) Identification of the active region of the DNA synthesis inhibitory gene p21Sdi1/CIP1/WAF1. EMBO J 14:555-63

Aggarwal, B B; Totpal, K; LaPushin, R et al. (1995) Diminished responsiveness of senescent normal human fibroblasts to TNF-dependent proliferation and interleukin production is not due to its effect on the receptors or on the activation of a nuclear factor NF-kappa B. Exp Cell Res 218:381-8