The main function of the Core is to provide state-of-the-art analytic methods to advance the proposed research in all three projects of the Program Project. We will provide reliable measurements and analyses of blood glucoregulatory, appetitive, and circadian hormones and other metabolite from human subjects who are affected by aging and/or sleep duration and quality in Projects 1 and 2. Assays will include leptin, ghrelin, gastric inhibitory polypeptide (GIP), C-peptide, cortisol, melatonin (from blood and saliva), 6-sulfatoxymelatonin (MTS6 in urine), glucose, insulin, lactate, pyruvate, free fatty acids (FFA), triglycerides, LDL, HDL, HbA1c, and NAD+. We will all also provide metabolic phenotyping methods to study tissue-specific and cellular metabolic flux in both the animal tissues and human blood samples and biopsies. The analytical procedures include high- performance liquid chromatography and mass spectrometry for metabolite analyses, cellular bioenergetics, real time monitoring of cellular redox state, biochemical analysis of sirtuin activity, gene expression analysis using quantitative PCR methods, as well as transcriptome analyses using next-generation sequencing for animal models in Project 3. All analytical methods have been validated and operational in our laboratory. The Metabolic and Molecular Core will provide critical support for the implementation of all Projects, explore potential use of novel analytical techniques, and will be closely integrated with the Analysis Core to ensure timely and safe archival of laboratory data.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Research Program Projects (P01)
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Special Emphasis Panel (ZAG1)
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Northwestern University at Chicago
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Baron, Kelly Glazer; Reid, Kathryn J; Malkani, Roneil G et al. (2017) Sleep Variability Among Older Adults With Insomnia: Associations With Sleep Quality and Cardiometabolic Disease Risk. Behav Sleep Med 15:144-157
Peek, Clara Bien; Levine, Daniel C; Cedernaes, Jonathan et al. (2017) Circadian Clock Interaction with HIF1? Mediates Oxygenic Metabolism and Anaerobic Glycolysis in Skeletal Muscle. Cell Metab 25:86-92
Mokhlesi, Babak; Grimaldi, Daniela; Beccuti, Guglielmo et al. (2017) Effect of one week of CPAP treatment of obstructive sleep apnoea on 24-hour profiles of glucose, insulin and counter-regulatory hormones in type 2 diabetes. Diabetes Obes Metab 19:452-456
Bass, Joseph T (2017) The circadian clock system's influence in health and disease. Genome Med 9:94
Fan, Emily P; Abbott, Sabra M; Reid, Kathryn J et al. (2017) Abnormal environmental light exposure in the intensive care environment. J Crit Care 40:11-14
Santostasi, Giovanni; Malkani, Roneil; Riedner, Brady et al. (2016) Phase-locked loop for precisely timed acoustic stimulation during sleep. J Neurosci Methods 259:101-114
Broussard, Josiane L; Wroblewski, Kristen; Kilkus, Jennifer M et al. (2016) Two Nights of Recovery Sleep Reverses the Effects of Short-term Sleep Restriction on Diabetes Risk. Diabetes Care 39:e40-1
Grimaldi, Daniela; Carter, Jason R; Van Cauter, Eve et al. (2016) Adverse Impact of Sleep Restriction and Circadian Misalignment on Autonomic Function in Healthy Young Adults. Hypertension 68:243-50
Mokhlesi, Babak; Grimaldi, Daniela; Beccuti, Guglielmo et al. (2016) Effect of One Week of 8-Hour Nightly Continuous Positive Airway Pressure Treatment of Obstructive Sleep Apnea on Glycemic Control in Type 2 Diabetes: A Proof-of-Concept Study. Am J Respir Crit Care Med 194:516-9
Broussard, Josiane L; Kilkus, Jennifer M; Delebecque, Fanny et al. (2016) Elevated ghrelin predicts food intake during experimental sleep restriction. Obesity (Silver Spring) 24:132-8

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