Apolipoprotein E (apoE) receptors, members of the low-density lipoprotein receptor (LDLR) family, contain common structure modules and share sequence homologue. One of the major challenges in the field of apoE receptor biology is to develop receptor-specific reagents including antibodies and cell lines. In the past, unique and specific reagents for apoE/apoE receptors have been developed by the labs of Drs. Bu, LaDu, Rebeck, Estus, and Weeber, and many of these reagents have been validated during the preliminary stage of this program project. The overall goal of the Molecular Cell Biology (MCB) Core is to facilitate studies in this program project by developing, organizing, and distributing apoE/apoE receptor reagents, and to carry out essential assays pertinent to our proposed studies. The five specific aims of the MCB Core are: 1) to develop and store apoE and apoE receptor-specific antibodies, cDNAs, cell lines, and siRNAs;2) to distribute these reagents and protocols to individual labs as they become available or needed;3) to perform standardized assays for apoE, apoE receptors, amyloid p-peptide (Ap), and Ap plaques;4) to make two new transgenic mouse lines and to assist individual projects to study the in vivo role of apoE receptors in these new and existing transgenic and knockout mice;and 5) to collaborate with individual projects to study apoE receptor proteolysis in Alzheimer's disease brains. The specific plans for the MCB Core include a designated technical personnel under the guidance of Dr. Bu to develop, organize, and supervise this Core facility and an easily accessible database documenting the available reagents and the protocols using them. Dr. Bu and the technical personnel will also provide consultations for the end users for technical issues and trouble shooting. These Core services will allow all individual projects to have access to our unique collection of apoE/apoE receptor reagents and to several standardized assays. It is anticipated that each of the five projects will utilize reagents and services from the MCB Core.

National Institute of Health (NIH)
National Institute on Aging (NIA)
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University of Illinois at Chicago
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Zhao, Jing; Fu, Yuan; Liu, Chia-Chen et al. (2014) Retinoic acid isomers facilitate apolipoprotein E production and lipidation in astrocytes through the retinoid X receptor/retinoic acid receptor pathway. J Biol Chem 289:11282-92
Liu, Chia-Chen; Kanekiyo, Takahisa; Roth, Barbara et al. (2014) Tyrosine-based signal mediates LRP6 receptor endocytosis and desensitization of Wnt/?-catenin pathway signaling. J Biol Chem 289:27562-70
Divekar, Shailaja D; Burrell, Teal C; Lee, Jennifer E et al. (2014) Ligand-induced homotypic and heterotypic clustering of apolipoprotein E receptor 2. J Biol Chem 289:15894-903
Tamboli, Irfan Y; Heo, Dongeun; Rebeck, G William (2014) Extracellular proteolysis of apolipoprotein E (apoE) by secreted serine neuronal protease. PLoS One 9:e93120
Tai, Leon M; Koster, Kevin P; Luo, Jia et al. (2014) Amyloid-? pathology and APOE genotype modulate retinoid X receptor agonist activity in vivo. J Biol Chem 289:30538-55
Kunzler, Jacqueline; Youmans, Katherine L; Yu, Chunjiang et al. (2014) APOE modulates the effect of estrogen therapy on A* accumulation EFAD-Tg mice. Neurosci Lett 560:131-6
Gold, Brian T; Zhu, Zude; Brown, Christopher A et al. (2014) White matter integrity is associated with cerebrospinal fluid markers of Alzheimer's disease in normal adults. Neurobiol Aging 35:2263-71
Kanekiyo, Takahisa; Xu, Huaxi; Bu, Guojun (2014) ApoE and A? in Alzheimer's disease: accidental encounters or partners? Neuron 81:740-54
Rodriguez, Gustavo A; Tai, Leon M; LaDu, Mary Jo et al. (2014) Human APOE4 increases microglia reactivity at A? plaques in a mouse model of A? deposition. J Neuroinflammation 11:111
Nwabuisi-Heath, Evelyn; Rebeck, G William; Ladu, Mary Jo et al. (2014) ApoE4 delays dendritic spine formation during neuron development and accelerates loss of mature spines inýývitro. ASN Neuro 6:e00134

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