To determine the impact of an experimental intervention on aging, it is essential that an investigator have knowledge of how the intervention alters the pathological lesions that occur with age. Age-related pathology increases exponentially with advancing age and is largely responsible for age-related morbidity as well as mortality. Pathological information also provides investigators with insight into the potential biological/molecular mechanism(s) of the intervention. Furthermore, the pathological assessment of old animals that are included in basic studies of biological aging processes is necessary to help investigators determine whether the changes in physiological/biochemical parameters measured are associated with or are independent of underlying pathological conditions. Thus it is essential to obtain accurate and thorough pathological assessments of aging animals. The Pathology Core will provide investigators in the three Projects with detailed end-of-life pathological analyses of the lesions that occur with age in colonies of tissue-specific (liver, skeletal muscle and white adipose tissue [WAT]) GHRKO mice . The Pathology Core will also provide investigators in the four Projects with cross-sectional pathological analyses of the specific tissues obtained from Ames dwarf, GHRKO and tissue-specific GHRKO mice.
The Specific Aims of the Pathology Core are as follows:
Aim 1 : To conduct comprehensive pathological analyses of the genetically manipulated mice and their wild- type littermates that die spontaneously or are in the moribund category in the aging colonies.
Aim 2 : To conduct cross-sectional histopathological analyses of tissues obtained from genetically manipulated mice and their littermates. These data will allow investigators to evaluate the effect of the genetic manipulations on the histological changes in specific tissues at specific age.

Public Health Relevance

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG031736-05
Application #
8448202
Study Section
Special Emphasis Panel (ZAG1-ZIJ-5)
Project Start
Project End
2015-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
5
Fiscal Year
2013
Total Cost
$194,429
Indirect Cost
$15,862
Name
Southern Illinois University School of Medicine
Department
Type
DUNS #
038415006
City
Springfield
State
IL
Country
United States
Zip Code
62794
Dominick, Graham; Bowman, Jacqueline; Li, Xinna et al. (2017) mTOR regulates the expression of DNA damage response enzymes in long-lived Snell dwarf, GHRKO, and PAPPA-KO mice. Aging Cell 16:52-60
Saccon, Tatiana D; Moreira, Fabiana; Cruz, Luis A et al. (2017) Ovarian aging and the activation of the primordial follicle reserve in the long-lived Ames dwarf and the short-lived bGH transgenic mice. Mol Cell Endocrinol 455:23-32
Hascup, Kevin N; Lynn, Mary K; Fitzgerald, Patrick J et al. (2017) Enhanced Cognition and Hypoglutamatergic Signaling in a Growth Hormone Receptor Knockout Mouse Model of Successful Aging. J Gerontol A Biol Sci Med Sci 72:329-337
Schneider, Augusto; Matkovich, Scot J; Saccon, Tatiana et al. (2017) Ovarian transcriptome associated with reproductive senescence in the long-living Ames dwarf mice. Mol Cell Endocrinol 439:328-336
Fang, Yimin; McFadden, Samuel; Darcy, Justin et al. (2017) Differential effects of early-life nutrient restriction in long-lived GHR-KO and normal mice. Geroscience 39:347-356
Bartke, Andrzej; Darcy, Justin (2017) GH and ageing: Pitfalls and new insights. Best Pract Res Clin Endocrinol Metab 31:113-125
Xu, Ming; Tchkonia, Tamar; Kirkland, James L (2016) Perspective: Targeting the JAK/STAT pathway to fight age-related dysfunction. Pharmacol Res 111:152-154
Hascup, Erin R; Wang, Feiya; Kopchick, John J et al. (2016) Inflammatory and Glutamatergic Homeostasis Are Involved in Successful Aging. J Gerontol A Biol Sci Med Sci 71:281-9
Bartke, A; Sun, L; Fang, Y et al. (2016) Growth hormone actions during development influence adult phenotype and longevity. Exp Gerontol 86:22-27
Matzkin, MarĂ­a Eugenia; Miquet, Johanna Gabriela; Fang, Yimin et al. (2016) Alterations in oxidative, inflammatory and apoptotic events in short-lived and long-lived mice testes. Aging (Albany NY) 8:95-110

Showing the most recent 10 out of 138 publications