The Analytical Imaging Core (Core B) will continue serving the four projects of this Program Project. The long-term goal of this core is to provide support for the needs of the individual projects on image-based procedures, protein analysis and chemical biology.
The specific aims of this core are: 1) to provide a comprehensive imaging facility that will assist the investigators in the morphological studies required to analyze the role of age-related changes in autophagy in different cellular and animal models;2) to work with the members of the individual projects toward the implementations in their experimental settings of methods for protein analysis;and 3) to initiate the development of targeted therapeutics based on modulation of autophagic pathways for testing in the different cellular and animal models used by the projects. The components of the core are: 1) The Imaging unit: That has and will continue providing advice on sample preparation/processing and techniques and procedures for analytical imaging, performing the specialized imaging procedures, and assisting with the interpretation of morphological images;2) The innovation unit: That explores, tests and implements procedures for protein modification analysis and for the development of chemical modulators of autophagy using structural and chemical biology. The services offered by the core include: a comprehensive array of analytic imaging procedures (direct and indirect immunofluorescence, real time fluorescence microscopy, confocal fluorescence, conventional and cryoelectron microscopy and immunogold), access to the imaging instrumentation, and a stock of common reagents (antibodies, probes and fluorescent dies) for the imaging procedures. The new services available during this period will include tomography, Immunogold/silver enhancement for intact cells labelling, in vivo imaging, protein aggregates islation and development, characterization and distribution of CMA activators. The key personnel are the directors, a dedicated technician, the expert technical staff of the imaging core at Einstein, an expert in chemical medicine and an expert in targeted therapeutics. The directors will supervise all of the activities of the core, offer advice and assist with result interpretation. The technical staff will prepare and process samples for morphological studies and maintain the supplies and reagents required for the core. Relevance: Imaging procedures and protein analysis are essential and common to all four projects in this program as they allow the study of the cellular process of interest in the whole cells or organs. The centralization of the procedures optimizes cost efficiency, guarantees their uniformity and allows for integration of the data obtained by the different groups. The incorporation of a chemical biology component to the core will facilitate development of anti-aging therapeutics based on autophagy modulation.

Public Health Relevance

The activities of this core are essential to create the basis of future efforts to translate the findings of the projects in this PP to the clinic. The chemical biology component of the core will facilitate development of anti-aging therapeutics based on autophagy modulation. The image-base procedures to track autophagy in vivo developed by this Core will permit to determine the efficacy of the different therapeutic interventions and may set the bases for future diagnosis tools.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
2P01AG031782-06A1
Application #
8739815
Study Section
Special Emphasis Panel (ZAG1)
Project Start
Project End
Budget Start
2014-09-15
Budget End
2015-04-30
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
City
Bronx
State
NY
Country
United States
Zip Code
10461
Batista-Gonzalez, Ana; Singh, Rajat (2017) Lysosomal function in ?-cell survival during glucolipotoxicity. Ann Transl Med 5:471
Satori, Chad P; Ramezani, Marzieh; Koopmeiners, Joseph S et al. (2017) Checkpoints for Preliminary Identification of Small Molecules found Enriched in Autophagosomes and Activated Mast Cell Secretions Analyzed by Comparative UPLC/MSe. Anal Methods 9:46-54
Raz, Yotam; Guerrero-Ros, Ignacio; Maier, Andrea et al. (2017) Activation-Induced Autophagy Is Preserved in CD4+ T-Cells in Familial Longevity. J Gerontol A Biol Sci Med Sci 72:1201-1206
Martinez-Lopez, Nuria; Tarabra, Elena; Toledo, Miriam et al. (2017) System-wide Benefits of Intermeal Fasting by Autophagy. Cell Metab 26:856-871.e5
Maus, Mate; Cuk, Mario; Patel, Bindi et al. (2017) Store-Operated Ca2+ Entry Controls Induction of Lipolysis and the Transcriptional Reprogramming to Lipid Metabolism. Cell Metab 25:698-712
Klionsky, Daniel J (see original citation for additional authors) (2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12:1-222
Martinez-Lopez, Nuria; Singh, Rajat (2016) Telemetric control of peripheral lipophagy by hypothalamic autophagy. Autophagy 12:1404-5
Martinez-Lopez, Nuria; Garcia-Macia, Marina; Sahu, Srabani et al. (2016) Autophagy in the CNS and Periphery Coordinate Lipophagy and Lipolysis in the Brown Adipose Tissue and Liver. Cell Metab 23:113-27
Champa, Devora; Orlacchio, Arturo; Patel, Bindi et al. (2016) Obatoclax kills anaplastic thyroid cancer cells by inducing lysosome neutralization and necrosis. Oncotarget 7:34453-71
Kaushik, Susmita; Cuervo, Ana Maria (2016) AMPK-dependent phosphorylation of lipid droplet protein PLIN2 triggers its degradation by CMA. Autophagy 12:432-8

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