Research in the major genetic model systems has revealed a strong and consistent association between dietary restriction, mutations that extend life span, and resistance to multiple stresses. Here we propose to investigate the mechanisms of aging with focus on starvation-dependent protection against oxidative damage and life span. We propose to continue our work to establish which combination of treatments and genetic manipulations causes the maximum resistance to toxins and identify the underiying mechanisms with focus on ER stress. We will continue and expand our mammalian cell and mouse studies to determine the role of short-term starvation on cellular senescence and aging in murine models and identify the mechanisms of starvation-dependent protection. We will also test the hypothesis that pathways analogous to those identified in yeast can protect normal but not cells with constitutively active pro-aging pathways (cancer cells) against oxidative damage and chemotherapy (Differential Stress Resistance, DSR) and study the mechanisms involved. Because older subjects are particularly sensitive to toxins, we will study DSR in young and old mice to identify interventions that can protect old organisms against cytotoxicity. The collaborations with Cohen and Lee will continue to introduce novel ideas, approaches, and research tools to our laboratory and provide the synergism necessary to accelerate our research. This collaborative biogerontology-based approach has the potential to identify new genetic pathways and mechanisms relevant to the basic biology of aging but also interventions that can be applied to the development of improved treatments and prevention of age-related diseases.

Public Health Relevance

Research on age-related diseases focuses primarily on the damaged cells or tissues affected by the disease. Here we propose to take a biogerontology-based approach to instead focus on the mechanisms of protection of all the healthy cells ofthe organism. These studies will help identify strategies, genetic pathways and drug targets to protect the organism against the age-dependent damage and diseases caused by endogenous as well as exogenous toxins.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG034906-04
Application #
8643560
Study Section
Special Emphasis Panel (ZAG1-ZIJ-5)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
4
Fiscal Year
2014
Total Cost
$426,332
Indirect Cost
$139,210
Name
University of Southern California
Department
Type
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
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Hu, Jia; Wei, Min; Mirzaei, Hamed et al. (2014) Tor-Sch9 deficiency activates catabolism of the ketone body-like acetic acid to promote trehalose accumulation and longevity. Aging Cell 13:457-67
Levine, Morgan E; Suarez, Jorge A; Brandhorst, Sebastian et al. (2014) Low protein intake is associated with a major reduction in IGF-1, cancer, and overall mortality in the 65 and younger but not older population. Cell Metab 19:407-17
Verghese, Joe; Annweiler, Cedric; Ayers, Emmeline et al. (2014) Motoric cognitive risk syndrome: multicountry prevalence and dementia risk. Neurology 83:718-26

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