Abstract

The main function of the Bone Stem Cell Core (Core C) is to isolate and distribute bone marrow- derived mesenchymal stem/progenitor cells (BMSCs) to the investigators of the individual projects, and to provide technical support for use of the cells. A key component essential to the success of the proposed Program Project is the availability of BMSCs because all projects require these cells. Thus, a centralized Core facility for the production of quality cells is crucial to the success of this dynamically coordinated Program Project. This Core will benefit the research efforts of individual investigator by reducing costs, providing quality control for study procedures, and enhancing collaborations among multiple disciplines to achieve greater scientific gain through an integrated approach. In addition, production and distribution of cells from a centralized facility will ensure that all studies start with cells of the same lineages and background.
The Specific Aims are: 1. Isolate, characterize and distribute mouse BMSCs, human bone marrow MSCs (hMSCs), and 2. Provide ongoing technical support for hypoxic and conventional MSC culture and differentiation (osteogenic, adipogenic, and chondrogenic) assays. By achieving these objectives, this Core will function as a unique resource to provide mouse and human BMSCs and technical assistance to the individual projects, as well as to other Georgia Regents University investigators who are interested in studies related to aging, metabolic bone diseases, or stem cell research.

Public Health Relevance

The main purpose of the Bone Stem Cell Core (Core C) is to generate mouse and human bone marrow- derived mesenchymal stem cells (BMSCs) for the Program Project, and provide advice to individual projects in using these cells. The Core C is essential for the success of the Program Project as all individual projects in the Program will need to use the same cells in order for the data sets generated from individual projects are comparable, consistent and synergistic.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
2P01AG036675-06A1
Application #
9209554
Study Section
Special Emphasis Panel (ZAG1-ZIJ-7 (O2))
Project Start
2011-05-01
Project End
2022-03-31
Budget Start
2016-09-01
Budget End
2017-08-31
Support Year
6
Fiscal Year
2017
Total Cost
$249,282
Indirect Cost
$85,281

  Institution

Name
Augusta University
Department
Type
Domestic Higher Education
DUNS #
City
Augusta
State
GA
Country
United States
Zip Code
30912

  Publications

Kolhe, Ravindra; Mondal, Ashis K; Pundkar, Chetan et al. (2018) Modulation of miRNAs by Vitamin C in Human Bone Marrow Stromal Cells. Nutrients 10:
Yu, Kanglun; Sellman, David P; Bahraini, Anoosh et al. (2018) Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone. J Orthop Res 36:653-662
Kesterke, Matthew J; Judd, Margaret A; Mooney, Mark P et al. (2018) Maternal environment and craniofacial growth: geometric morphometric analysis of mandibular shape changes with in utero thyroxine overexposure in mice. J Anat 233:46-54
Howie, R Nicole; Herberg, Samuel; Durham, Emily et al. (2018) Selective serotonin re-uptake inhibitor sertraline inhibits bone healing in a calvarial defect model. Int J Oral Sci 10:25
Elsayed, Ranya; Abraham, Pheba; Awad, Mohamed E et al. (2018) Removal of matrix-bound zoledronate prevents post-extraction osteonecrosis of the jaw by rescuing osteoclast function. Bone 110:141-149
Murphy, Cameron; Withrow, Joseph; Hunter, Monte et al. (2018) Emerging role of extracellular vesicles in musculoskeletal diseases. Mol Aspects Med 60:123-128
Roser-Page, Susanne; Vikulina, Tatyana; Yu, Kanglun et al. (2018) Neutralization of CD40 ligand costimulation promotes bone formation and accretion of vertebral bone mass in mice. Rheumatology (Oxford) 57:1105-1114
Bollag, Wendy B; Choudhary, Vivek; Zhong, Qing et al. (2018) Deletion of protein kinase D1 in osteoprogenitor cells results in decreased osteogenesis in vitro and reduced bone mineral density in vivo. Mol Cell Endocrinol 461:22-31
Kim, Beom-Jun; Lee, Seung Hun; Kwak, Mi Kyung et al. (2018) Inverse relationship between serum hsCRP concentration and hand grip strength in older adults: a nationwide population-based study. Aging (Albany NY) 10:2051-2061
Kim, B-J; Lee, S H; Isales, C M et al. (2018) The positive association of total protein intake with femoral neck strength (KNHANES IV). Osteoporos Int 29:1397-1405

Showing the most recent 10 out of 51 publications