The goal of the Muscle/Bone Phenotyping Core is to support the research alms of all subprojects within the program project. The overall aim of the program project is to understand the mechanisms underlying crosstalk between muscle and bone that may contribute to the age related decline In muscle and bone mass and function. This global question is addressed by each subproject from a different perspective, including: the effects of muscle on osteoblast and osteocyte function with aging (subproject 1);osteocyte modulation of muscle function during aging (subproject 2);osteocyte control of osteoblast dynamics with aging (subproject 3);and effects of aging on osteocyte responses to mechanical stimulation (subproject 4). The Muscle/Bone Phenotyping Core will provide phenotyping support for all subprojects, which will include In vivo and ex vivo X-ray, densitometric and microCT analysis of skeletal tissues, histological preparation and staining of muscle and mineralized tissues, quantitative histomorphometry and dynamic bone and muscle histomorphometry, immunohistochemistry and in situ hybridization. These techniques are essential for all the subprojects in which muscle and bone phenotypes are being characterized as a function of age in transgenic mouse models with altered osteocyte or muscle function, as well as mice that have been subjected to mechanical loading. In addition to providing these services the core will provide standardized protocols for sample preparation and for all methods within the core to maintain consistency across the subprojects. The core will also act as a resource to provide training in the above techniques for students, postdocs and research personnel conducting research within subprojects 1, 2, 3 and 4. By centralizing these phenotyping methods within a single core, we will standardize these techniques across all the subprojects, accelerate the pace of the research, enhance the education of students and postdocs and enable the research to be completed in a more cost-effective manner.

Public Health Relevance

This research core will provide centralized support for four subprojects within a program project whose goal is to understand the mechanisms underlying muscle and bone interactions that contribute to age-related decline in muscle and bone mass. The core will standardize methods for analysis of muscle and bone in the mouse models used in all projects. This research may lead to new treatments for osteopenia/sarcopenla.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG039355-02
Application #
8460474
Study Section
Special Emphasis Panel (ZAG1-ZIJ-9)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
2
Fiscal Year
2013
Total Cost
$203,584
Indirect Cost
$67,862
Name
University of Missouri Kansas City
Department
Type
DUNS #
010989619
City
Kansas City
State
MO
Country
United States
Zip Code
64110
Bonewald, Lynda (2018) Use it or lose it to age: A review of bone and muscle communication. Bone 120:212-218
Kitase, Yukiko; Vallejo, Julian A; Gutheil, William et al. (2018) ?-aminoisobutyric Acid, l-BAIBA, Is a Muscle-Derived Osteocyte Survival Factor. Cell Rep 22:1531-1544
Pin, Fabrizio; Barreto, Rafael; Kitase, Yukiko et al. (2018) Growth of ovarian cancer xenografts causes loss of muscle and bone mass: a new model for the study of cancer cachexia. J Cachexia Sarcopenia Muscle 9:685-700
Morris, Josephine L; Cross, Stephen J; Lu, Yinhui et al. (2018) Live imaging of collagen deposition during skin development and repair in a collagen I - GFP fusion transgenic zebrafish line. Dev Biol 441:4-11
Tiede-Lewis, LeAnn M; Xie, Yixia; Hulbert, Molly A et al. (2017) Degeneration of the osteocyte network in the C57BL/6 mouse model of aging. Aging (Albany NY) 9:2190-2208
Wang, Zhiying; Bian, Liangqiao; Mo, Chenglin et al. (2017) Targeted quantification of lipid mediators in skeletal muscles using restricted access media-based trap-and-elute liquid chromatography-mass spectrometry. Anal Chim Acta 984:151-161
Jähn, Katharina; Kelkar, Shilpa; Zhao, Hong et al. (2017) Osteocytes Acidify Their Microenvironment in Response to PTHrP In Vitro and in Lactating Mice In Vivo. J Bone Miner Res 32:1761-1772
Huang, Jian; Romero-Suarez, Sandra; Lara, Nuria et al. (2017) Crosstalk between MLO-Y4 osteocytes and C2C12 muscle cells is mediated by the Wnt/?-catenin pathway. JBMR Plus 1:86-100
Bonewald, Lynda F (2017) The Role of the Osteocyte in Bone and Nonbone Disease. Endocrinol Metab Clin North Am 46:1-18
Vemula, Harika; Kitase, Yukiko; Ayon, Navid J et al. (2017) Gaussian and linear deconvolution of LC-MS/MS chromatograms of the eight aminobutyric acid isomers. Anal Biochem 516:75-85

Showing the most recent 10 out of 39 publications