The goal of the Muscle/Bone Phenotyping Core is to support the research alms of all subprojects within the program project. The overall aim of the program project is to understand the mechanisms underlying crosstalk between muscle and bone that may contribute to the age related decline In muscle and bone mass and function. This global question is addressed by each subproject from a different perspective, including: the effects of muscle on osteoblast and osteocyte function with aging (subproject 1);osteocyte modulation of muscle function during aging (subproject 2);osteocyte control of osteoblast dynamics with aging (subproject 3);and effects of aging on osteocyte responses to mechanical stimulation (subproject 4). The Muscle/Bone Phenotyping Core will provide phenotyping support for all subprojects, which will include In vivo and ex vivo X-ray, densitometric and microCT analysis of skeletal tissues, histological preparation and staining of muscle and mineralized tissues, quantitative histomorphometry and dynamic bone and muscle histomorphometry, immunohistochemistry and in situ hybridization. These techniques are essential for all the subprojects in which muscle and bone phenotypes are being characterized as a function of age in transgenic mouse models with altered osteocyte or muscle function, as well as mice that have been subjected to mechanical loading. In addition to providing these services the core will provide standardized protocols for sample preparation and for all methods within the core to maintain consistency across the subprojects. The core will also act as a resource to provide training in the above techniques for students, postdocs and research personnel conducting research within subprojects 1, 2, 3 and 4. By centralizing these phenotyping methods within a single core, we will standardize these techniques across all the subprojects, accelerate the pace of the research, enhance the education of students and postdocs and enable the research to be completed in a more cost-effective manner.

Public Health Relevance

This research core will provide centralized support for four subprojects within a program project whose goal is to understand the mechanisms underlying muscle and bone interactions that contribute to age-related decline in muscle and bone mass. The core will standardize methods for analysis of muscle and bone in the mouse models used in all projects. This research may lead to new treatments for osteopenia/sarcopenla.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Research Program Projects (P01)
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Special Emphasis Panel (ZAG1-ZIJ-9)
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University of Missouri Kansas City
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Bonewald, Lynda (2018) Use it or lose it to age: A review of bone and muscle communication. Bone 120:212-218
Kitase, Yukiko; Vallejo, Julian A; Gutheil, William et al. (2018) ?-aminoisobutyric Acid, l-BAIBA, Is a Muscle-Derived Osteocyte Survival Factor. Cell Rep 22:1531-1544
Pin, Fabrizio; Barreto, Rafael; Kitase, Yukiko et al. (2018) Growth of ovarian cancer xenografts causes loss of muscle and bone mass: a new model for the study of cancer cachexia. J Cachexia Sarcopenia Muscle 9:685-700
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Bonewald, Lynda F (2017) The Role of the Osteocyte in Bone and Nonbone Disease. Endocrinol Metab Clin North Am 46:1-18
Vemula, Harika; Kitase, Yukiko; Ayon, Navid J et al. (2017) Gaussian and linear deconvolution of LC-MS/MS chromatograms of the eight aminobutyric acid isomers. Anal Biochem 516:75-85

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