Abstract

The overall goal of the Immunology Core is to provide a central resource for uniform measurement and analysis of cellular and humoral immune responses. The Core will provide all immunologic testing for mice and will assist with detailed characterization of vaccine-induced immune responses in rhesus macaques. Immunologic assays that will be routinely performed include ELISA, neutralization assays and immunoprecipitation Western Blot for analysis of humoral immunity. Cellular immunity will be evaluated by ELISPOT assays combined with flow cytometric analysis of MHC tetramer responses and cytokine-secreting antigen-specific T cells. In addition to routine testing, the Immunology Core will also assist in the planning and development of new assays to assess the functional heterogeneity of vaccine-induced T cells in comparison to infection-induced T cells, provide training for isolation of mucosal T lymphocytes from the genital mucosa in mice, help in the analysis, interpretation and presentation of immunologic data, and serve as a repository for all immunologic reagents used in both projects. The standardized testing provided by the core will facilitate comparisons across different experiments and between projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
2P01AI046006-06A1
Application #
7006731
Study Section
Special Emphasis Panel (ZAI1-EC-A (M2))
Project Start
2005-08-01
Project End
2010-03-31
Budget Start
2005-08-01
Budget End
2006-03-31
Support Year
6
Fiscal Year
2005
Total Cost
$179,644
Indirect Cost

  Institution

Name
Harvard University
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Meythaler, Mareike; Wang, Zichun; Martinot, Amanda et al. (2011) Early induction of polyfunctional simian immunodeficiency virus (SIV)-specific T lymphocytes and rapid disappearance of SIV from lymph nodes of sooty mangabeys during primary infection. J Immunol 186:5151-61
Reszka, Natalia; Zhou, Changhong; Song, Byeongwoon et al. (2010) Simian TRIM5alpha proteins reduce replication of herpes simplex virus. Virology 398:243-50
Meythaler, Mareike; Martinot, Amanda; Wang, Zichun et al. (2009) Differential CD4+ T-lymphocyte apoptosis and bystander T-cell activation in rhesus macaques and sooty mangabeys during acute simian immunodeficiency virus infection. J Virol 83:572-83
Liu, Xueqiao; Broberg, Eeva; Watanabe, Daisuke et al. (2009) Genetic engineering of a modified herpes simplex virus 1 vaccine vector. Vaccine 27:2760-7
Cliffe, Anna R; Garber, David A; Knipe, David M (2009) Transcription of the herpes simplex virus latency-associated transcript promotes the formation of facultative heterochromatin on lytic promoters. J Virol 83:8182-90
Meythaler, Mareike; Pryputniewicz, Sarah; Kaur, Amitinder (2008) Kinetics of T lymphocyte apoptosis and the cellular immune response in SIVmac239-infected rhesus macaques. J Med Primatol 37 Suppl 2:33-45
Brockman, Mark A; Knipe, David M (2008) Herpes simplex virus as a tool to define the role of complement in the immune response to peripheral infection. Vaccine 26 Suppl 8:I94-9
Cliffe, Anna R; Knipe, David M (2008) Herpes simplex virus ICP0 promotes both histone removal and acetylation on viral DNA during lytic infection. J Virol 82:12030-8
Watanabe, Daisuke; Brockman, Mark A; Ndung'u, Thumbi et al. (2007) Properties of a herpes simplex virus multiple immediate-early gene-deleted recombinant as a vaccine vector. Virology 357:186-98
Kaur, Amitinder; Sanford, Hannah B; Garry, Deirdre et al. (2007) Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus. Virology 357:199-214

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