The Mouse and Transplantation Core will have overall responsibilities for training all Projects in the transplantation of skin allografts and monitoring for graft survival. It will also have responsibility for maintenance, treatment, and surgical manipulation of virus treated experimental animals in BSL2/3 level biocontainment. All Program Project experiments will utilize VAF animals housed in a VAF facility or in a limited access biocontainment facility designed to maintain the highest degree of cleanliness. The emphasis on strict limited-access biocontainment will preclude cross-contamination and corruption of outcomes by unintended infectious agents. The objectives and responsibilities of the Animal Core Unit are as follows: By administering or training personnel in proper administration of reagents, including viruses, anti-CDI 54 mAb, other monoclonal antibodies, cells, TLR agonists, and other specific agents to be developed in the course of the Program to animals in VAF and in biocontainment. By preparing hematopoietic "synchimeras," an animal model system that is crucial for the success of Projects 1-3. Procurement of cells, biopsy and autopsy specimens for histological and flow cytometric analysis from animals in biocontainment. Animal Core personnel will perform or assist with performing skin grafts for all Projects. Serological testing of experimental animals to document that infection has occurred and that there is no cross-contamination. In brief, the Mouse and Transplantation Core will provide essential assistance and personnel training to each Project with the management and execution of in vivo studies conducted in defined VAF facilities and in strictly controlled infectious conditions in biocontainment.

Public Health Relevance

The Animal Core will provide essential service to all of the Projects in the Program regarding animal handling, use of infectious agents in animals, and transplantation procedures. Transplantation of islets and skin are specialized procedures that are used in this Program to study how viruses and new drugs affect graft survival in patients in the clinic. These transplantation techniques must be standardized between laboratories for evaluation of the information regarding virus interaction with drugs and graft suivival

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI046629-14
Application #
8661688
Study Section
Special Emphasis Panel (ZAI1-PTM-I)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
14
Fiscal Year
2014
Total Cost
$145,117
Indirect Cost
$56,900
Name
University of Massachusetts Medical School Worcester
Department
Type
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
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