The Flow Cytometry Core will provide essential expertise, support and reagents to program project investigators for quantitative and phenotypic analysis of complex lymphocyte and antigen-presenting cell populations. In addition, considerable expertise is available for the design and implementation of high- throughput cell sorting experiments. The Core director Dr. Aguila, currently Associate Director of the Flow Cytometry Facility at UCHC, trained at Stanford in stem cell isolation by flow cytometry and so is well-versed at analysis and cell sorting of rare populations. The Flow Cytometry Facility is also well-equipped to handle the complexity as well as the volume of samples generated in this Program, having 6 analyzers and two high-speed cell sorters with multicolor capabilities. The equipment includes three BD-LSR 1 machines with two able to perform up to 15-parameter analysis and the third a five laser modified machine to allow up to 21 parameter analysis incuding multiple lines for detection of quantum dot-type reagents. Two five laser FACSAria lis are available for high-speed multicolor sorting and analysis. One of these is housed in a Baker hood to allow sorting of potentially infected tissues from mice or humans. In addition to support for facility costs, the Core will also provide technical assistance for processing, cell sorting, and analyzing samples and data. Having a dedicated research assistant for the Program will ensure uniformity of quality and help to increase efficiency and productivity. For these reasons the Flow Cytometry Core continues to be an integral component of each project and is therefore requisite to the success of this Program.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI056172-09
Application #
8728383
Study Section
Special Emphasis Panel (ZAI1-PA-I)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
9
Fiscal Year
2014
Total Cost
$268,792
Indirect Cost
$78,228
Name
University of Connecticut
Department
Type
DUNS #
022254226
City
Farmington
State
CT
Country
United States
Zip Code
06030
Tsurutani, Naomi; Mittal, Payal; St Rose, Marie-Clare et al. (2016) Costimulation Endows Immunotherapeutic CD8 T Cells with IL-36 Responsiveness during Aerobic Glycolysis. J Immunol 196:124-34
Song, Jeongmin; Wilhelm, Cara L; Wangdi, Tamding et al. (2016) Absence of TLR11 in Mice Does Not Confer Susceptibility to Salmonella Typhi. Cell 164:827-8
Benoun, Joseph M; Labuda, Jasmine C; McSorley, Stephen J (2016) Collateral Damage: Detrimental Effect of Antibiotics on the Development of Protective Immune Memory. MBio 7:
Svedova, Julia; Tsurutani, Naomi; Liu, Wenhai et al. (2016) TNF and CD28 Signaling Play Unique but Complementary Roles in the Systemic Recruitment of Innate Immune Cells after Staphylococcus aureus Enterotoxin A Inhalation. J Immunol 196:4510-21
Romagnoli, Pablo A; Sheridan, Brian S; Pham, Quynh-Mai et al. (2016) IL-17A-producing resident memory γδ T cells orchestrate the innate immune response to secondary oral Listeria monocytogenes infection. Proc Natl Acad Sci U S A 113:8502-7
Cauley, Linda S (2016) Environmental cues orchestrate regional immune surveillance and protection by pulmonary CTLs. J Leukoc Biol 100:905-912
Romagnoli, P A; Fu, H H; Qiu, Z et al. (2016) Differentiation of distinct long-lived memory CD4 T cells in intestinal tissues after oral Listeria monocytogenes infection. Mucosal Immunol :
Cauley, Linda S; Vella, Anthony T (2015) Why is coinfection with influenza virus and bacteria so difficult to control? Discov Med 19:33-40
Colpitts, Sara L; Puddington, Lynn; Lefrançois, Leo (2015) IL-15 receptor α signaling constrains the development of IL-17-producing γδ T cells. Proc Natl Acad Sci U S A 112:9692-7
Pham, Oanh H; McSorley, Stephen J (2015) Protective host immune responses to Salmonella infection. Future Microbiol 10:101-10

Showing the most recent 10 out of 76 publications