The Flow Cytometry Core will provide essential expertise, support and reagents to program project investigators for quantitative and phenotypic analysis of complex lymphocyte and antigen-presenting cell populations. In addition, considerable expertise is available for the design and implementation of high- throughput cell sorting experiments. The Core director Dr. Aguila, currently Associate Director of the Flow Cytometry Facility at UCHC, trained at Stanford in stem cell isolation by flow cytometry and so is well-versed at analysis and cell sorting of rare populations. The Flow Cytometry Facility is also well-equipped to handle the complexity as well as the volume of samples generated in this Program, having 6 analyzers and two high-speed cell sorters with multicolor capabilities. The equipment includes three BD-LSR 1 machines with two able to perform up to 15-parameter analysis and the third a five laser modified machine to allow up to 21 parameter analysis incuding multiple lines for detection of quantum dot-type reagents. Two five laser FACSAria lis are available for high-speed multicolor sorting and analysis. One of these is housed in a Baker hood to allow sorting of potentially infected tissues from mice or humans. In addition to support for facility costs, the Core will also provide technical assistance for processing, cell sorting, and analyzing samples and data. Having a dedicated research assistant for the Program will ensure uniformity of quality and help to increase efficiency and productivity. For these reasons the Flow Cytometry Core continues to be an integral component of each project and is therefore requisite to the success of this Program.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1-PA-I)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Connecticut
United States
Zip Code
Atif, Shaikh M; Lee, Seung-Joo; Li, Lin-Xi et al. (2015) Rapid CD4+ T-cell responses to bacterial flagellin require dendritic cell expression of Syk and CARD9. Eur J Immunol 45:513-24
Nanton, Minelva R; Lee, Seung-Joo; Atif, Shaikh M et al. (2015) Direct visualization of endogenous Salmonella-specific B cells reveals a marked delay in clonal expansion and germinal center development. Eur J Immunol 45:428-41
O'Donnell, Hope; Pham, Oanh H; Li, Lin-xi et al. (2014) Toll-like receptor and inflammasome signals converge to amplify the innate bactericidal capacity of T helper 1 cells. Immunity 40:213-24
McSorley, Stephen J (2014) Immunity to intestinal pathogens: lessons learned from Salmonella. Immunol Rev 260:168-82
Sheridan, Brian S; Pham, Quynh-Mai; Lee, Young-Tae et al. (2014) Oral infection drives a distinct population of intestinal resident memory CD8(+) T cells with enhanced protective function. Immunity 40:747-57
Wu, Tao; Hu, Yinghong; Lee, Young-Tae et al. (2014) Lung-resident memory CD8 T cells (TRM) are indispensable for optimal cross-protection against pulmonary virus infection. J Leukoc Biol 95:215-24
Kumar, S; Colpitts, S L; Menoret, A et al. (2013) Rapid ** T-cell responses orchestrate innate immunity in response to Staphylococcal enterotoxin A. Mucosal Immunol 6:1006-15
Wright, Kyle T; Vella, Anthony T (2013) RKIP contributes to IFN-? synthesis by CD8+ T cells after serial TCR triggering in systemic inflammatory response syndrome. J Immunol 191:708-16
Blair, David A; Turner, Damian L; Bose, Tina O et al. (2011) Duration of antigen availability influences the expansion and memory differentiation of T cells. J Immunol 187:2310-21
Griffin, A J; McSorley, S J (2011) Development of protective immunity to Salmonella, a mucosal pathogen with a systemic agenda. Mucosal Immunol 4:371-82

Showing the most recent 10 out of 46 publications