To understand the immune response to Francisella tularensis (Ft), and its interaction with immune cells, histological and immunohistochemical analysis of cells and tissues, as well as an analysis of individual immune cell populations and signaling molecules produced, are required. Thus, the purpose of Core C is to provide the means and technological expertise for investigators to analyze immune cells, quantify signalling molecules (cytokines and intracellular signaling molecules), and histologically identify cells and tissues obtained from mice. This includes the use of immunohistochemical techniques to localize and identify antigens on the surface of, and inside immune cells. This Core facilitv will be composed of three primary components: (1) a Flow Cvtometrv service, (2) a Bio-Plex Multiplex Array (BMA) Protein Detection service. and (3) a Histochemistry/lmmunohistochemistry service. All three services are currently available, and being provided to P01 investigators on all 3 currently funded subprojects. In regard to the current proposal, Core C will continue to provide the above services to P01 investigators on all proposed subprojects. The function of the three Immunology Core components is indicated in more detail below: 1. Flow Cytometry Service: This component will provide investigators access to flow cytometry analysis and cell sorting for the purpose of analyzing individual immune cell populations, including surface marker expression and intracellular cytokine production. Specific functions will include: flow cytometer operation, investigator training, equipment maintainance, ordering of reagents and supplies;2. BMA Protein Detection Service: This component wi utilize an established BMA technology sold by BioRad to quantitate secreted cytokines and intracellular signalling molecules utilizing a Bio-Plex 200 instrument dedicated to this purpose. Specific functions wil| include: acquiring reagents and kits, performing assays, performing analysis of samples, assisting in data/assay interpretation when appropriate, and maintaining the required equipment;3. Histology/lmmunohistochemistry Service: This component will process cells and tissues provided by investigators for histologic and immunhistochemical evaluation. Functions will include tissue embedding and sectioning, histochemical and immunohistochemical staining of cells and tissues, equipment maintainance and assistance in the data interpretation. Core C will now also produce, test, and standardize inactivated Ft (iFO-mAb complexes, which will be used by all 3 subprojects.

Public Health Relevance

To understand the immune response to Francisella tularensis, and its interaction with immune cells, histological and immunohistochemical analysis of cells and tissues, as well as an analysis of individual immune cell populations and signaling molecules produced, is required. Core C will provide the means and technological expertise for investigators to analyze immune cells, quantify signalling molecules (cytokines and intracellular signaling molecules), and to histologically identify cells and tissues obtained from mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI056320-09
Application #
8698578
Study Section
Special Emphasis Panel (ZAI1-LR-M (S1))
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
9
Fiscal Year
2013
Total Cost
$237,673
Indirect Cost
$84,048
Name
Albany Medical College
Department
Type
DUNS #
190592162
City
Albany
State
NY
Country
United States
Zip Code
12208
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Sunagar, Raju; Kumar, Sudeep; Franz, Brian J et al. (2016) Tularemia vaccine development: paralysis or progress? Vaccine (Auckl) 6:9-23
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Franz, Brian J; Li, Ying; Bitsaktsis, Constantine et al. (2015) Downmodulation of vaccine-induced immunity and protection against the intracellular bacterium Francisella tularensis by the inhibitory receptor FcγRIIB. J Immunol Res 2015:840842
Bitsaktsis, Constantine; Babadjanova, Zulfia; Gosselin, Edmund J (2015) In vivo mechanisms involved in enhanced protection utilizing an Fc receptor-targeted mucosal vaccine platform in a bacterial vaccine and challenge model. Infect Immun 83:77-89
Suresh, Ragavan Varadharajan; Ma, Zhuo; Sunagar, Raju et al. (2015) Preclinical testing of a vaccine candidate against tularemia. PLoS One 10:e0124326

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