To understand the immune response to Francisella tularensis (Ft), and its interaction with immune cells, histological and immunohistochemical analysis of cells and tissues, as well as an analysis of individual immune cell populations and signaling molecules produced, are required. Thus, the purpose of Core C is to provide the means and technological expertise for investigators to analyze immune cells, quantify signalling molecules (cytokines and intracellular signaling molecules), and histologically identify cells and tissues obtained from mice. This includes the use of immunohistochemical techniques to localize and identify antigens on the surface of, and inside immune cells. This Core facilitv will be composed of three primary components: (1) a Flow Cvtometrv service, (2) a Bio-Plex Multiplex Array (BMA) Protein Detection service. and (3) a Histochemistry/lmmunohistochemistry service. All three services are currently available, and being provided to P01 investigators on all 3 currently funded subprojects. In regard to the current proposal, Core C will continue to provide the above services to P01 investigators on all proposed subprojects. The function of the three Immunology Core components is indicated in more detail below: 1. Flow Cytometry Service: This component will provide investigators access to flow cytometry analysis and cell sorting for the purpose of analyzing individual immune cell populations, including surface marker expression and intracellular cytokine production. Specific functions will include: flow cytometer operation, investigator training, equipment maintainance, ordering of reagents and supplies;2. BMA Protein Detection Service: This component wi utilize an established BMA technology sold by BioRad to quantitate secreted cytokines and intracellular signalling molecules utilizing a Bio-Plex 200 instrument dedicated to this purpose. Specific functions wil| include: acquiring reagents and kits, performing assays, performing analysis of samples, assisting in data/assay interpretation when appropriate, and maintaining the required equipment;3. Histology/lmmunohistochemistry Service: This component will process cells and tissues provided by investigators for histologic and immunhistochemical evaluation. Functions will include tissue embedding and sectioning, histochemical and immunohistochemical staining of cells and tissues, equipment maintainance and assistance in the data interpretation. Core C will now also produce, test, and standardize inactivated Ft (iFO-mAb complexes, which will be used by all 3 subprojects.

Public Health Relevance

To understand the immune response to Francisella tularensis, and its interaction with immune cells, histological and immunohistochemical analysis of cells and tissues, as well as an analysis of individual immune cell populations and signaling molecules produced, is required. Core C will provide the means and technological expertise for investigators to analyze immune cells, quantify signalling molecules (cytokines and intracellular signaling molecules), and to histologically identify cells and tissues obtained from mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI056320-09
Application #
8698578
Study Section
Special Emphasis Panel (ZAI1-LR-M (S1))
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
9
Fiscal Year
2013
Total Cost
$237,673
Indirect Cost
$84,048
Name
Albany Medical College
Department
Type
DUNS #
190592162
City
Albany
State
NY
Country
United States
Zip Code
12208
Dixon, Ann M; Drake, Lisa; Hughes, Kelly T et al. (2014) Differential transmembrane domain GXXXG motif pairing impacts major histocompatibility complex (MHC) class II structure. J Biol Chem 289:11695-703
Pham, Giang H; Iglesias, Bibiana V; Gosselin, Edmund J (2014) Fc receptor-targeting of immunogen as a strategy for enhanced antigen loading, vaccination, and protection using intranasally administered antigen-pulsed dendritic cells. Vaccine 32:5212-20
Furuya, Yoichi; Wijesundara, Danushka K; Neeman, Teresa et al. (2014) Use and misuse of statistical significance in survival analyses. MBio 5:e00904-14
Ma, Zhuo; Banik, Sukalyani; Rane, Harshita et al. (2014) EmrA1 membrane fusion protein of Francisella tularensis LVS is required for resistance to oxidative stress, intramacrophage survival and virulence in mice. Mol Microbiol 91:976-95
Singh, Anju; Rahman, Tabassum; Malik, Meenakshi et al. (2013) Discordant results obtained with Francisella tularensis during in vitro and in vivo immunological studies are attributable to compromised bacterial structural integrity. PLoS One 8:e58513
Furuya, Yoichi; Kirimanjeswara, Girish S; Roberts, Sean et al. (2013) Increased susceptibility of IgA-deficient mice to pulmonary Francisella tularensis live vaccine strain infection. Infect Immun 81:3434-41
Mahawar, Manish; Rabadi, Seham M; Banik, Sukalyani et al. (2013) Identification of a live attenuated vaccine candidate for tularemia prophylaxis. PLoS One 8:e61539
Metzger, Dennis W; Salmon, Sharon L; Kirimanjeswara, Girish (2013) Differing effects of interleukin-10 on cutaneous and pulmonary Francisella tularensis live vaccine strain infection. Infect Immun 81:2022-7
Iglesias, Bibiana V; Bitsaktsis, Constantine; Pham, Giang et al. (2013) Multiple mechanisms mediate enhanced immunity generated by mAb-inactivated F. tularensis immunogen. Immunol Cell Biol 91:139-48
Dotson, Rachel J; Rabadi, Seham M; Westcott, Elizabeth L et al. (2013) Repression of inflammasome by Francisella tularensis during early stages of infection. J Biol Chem 288:23844-57

Showing the most recent 10 out of 38 publications