Virology Core C is the central point of the Program Project for specimen handling, storage and distribution from the domestic Clinical Core B (Figure 1). Scientifically, it is devoted to definition and dissection of HIV-1 levels, resistance states and the anatomic locations of HIV among participants enrolled and followed in this Program. This core is essential to and tightly integrated with the other cores and projects. After delivery from the clinic the samples are stored in the repository and can be retrieved and routed to the requestor quickly. Viral load results are sent to Admin Core A and Clinical Core B. Currently, the repository occupies five -80 freezers and four LN2 tanks. While existing space can handle additional plasma samples, another LN2 tank is required to store new cell samples that will be collected. Much of the emphasis of our work over the last 5 years has been to define sites and mechanisms of HIV-1 persistence during the course of antiretroviral therapy. We were first to show that viral latency in T cells is established early after infection^ and one of the first groups to demonstrate that continued low-grade virus replication is confined to specific cell types during the course of HAART^'. Understanding what factors influence persistence of HIV-1 replication is an ongoing emphasis of our laboratories""""""""'^. Many of the specific aims in this program are directed at virological and immunological factors that influence HIV-1 dynamics. As such, we have spent considerable time developing quantitative, reproducible, and sensitive assays that can measure HIV-1 at different stages of the virus cycle and in a variety of tissues and cell types.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI057005-08
Application #
8379845
Study Section
Special Emphasis Panel (ZCA1-GRB-T)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
8
Fiscal Year
2012
Total Cost
$113,330
Indirect Cost
$45,059
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Patel, Rena C; Baeten, Jared M; Heffron, Renee et al. (2017) Brief Report: Hormonal Contraception Is Not Associated With Reduced ART Effectiveness Among Women Initiating ART: Evidence From Longitudinal Data. J Acquir Immune Defic Syndr 75:91-96
Obermeit, Lisa C; Beltran, Jessica; Casaletto, Kaitlin B et al. (2017) Evaluating the accuracy of self-report for the diagnosis of HIV-associated neurocognitive disorder (HAND): defining ""symptomatic"" versus ""asymptomatic"" HAND. J Neurovirol 23:67-78
Hu, Xintao; Valentin, Antonio; Rosati, Margherita et al. (2017) HIV Env conserved element DNA vaccine alters immunodominance in macaques. Hum Vaccin Immunother 13:2859-2871
Fife, Kenneth H; Mugwanya, Kenneth; Thomas, Katherine K et al. (2016) Transient Increase in Herpes Simplex Virus Type 2 (HSV-2)-Associated Genital Ulcers Following Initiation of Antiretroviral Therapy in HIV/HSV-2-Coinfected Individuals. J Infect Dis 213:1573-8
Smith, Kellie N; Mailliard, Robbie B; Piazza, Paolo A et al. (2016) Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells. MBio 7:
Hu, Xintao; Valentin, Antonio; Dayton, Frances et al. (2016) DNA Prime-Boost Vaccine Regimen To Increase Breadth, Magnitude, and Cytotoxicity of the Cellular Immune Responses to Subdominant Gag Epitopes of Simian Immunodeficiency Virus and HIV. J Immunol 197:3999-4013
Collier, Ann C; Chun, Tae-Wook; Maenza, Janine et al. (2016) A Pilot Study of Raltegravir Plus Combination Antiretroviral Therapy in Early Human Immunodeficiency Virus Infection: Challenges and Lessons Learned. Biores Open Access 5:15-21
Magaret, Amalia S; Mujugira, Andrew; Hughes, James P et al. (2016) Effect of Condom Use on Per-act HSV-2 Transmission Risk in HIV-1, HSV-2-discordant Couples. Clin Infect Dis 62:456-61
Johnston, Christine; Harrington, Robert; Jain, Rupali et al. (2016) Safety and Efficacy of Combination Antiretroviral Therapy in Human Immunodeficiency Virus-Infected Adults Undergoing Autologous or Allogeneic Hematopoietic Cell Transplantation for Hematologic Malignancies. Biol Blood Marrow Transplant 22:149-56
Ma, Qing; Vaida, Florin; Wong, Jenna et al. (2016) Long-term efavirenz use is associated with worse neurocognitive functioning in HIV-infected patients. J Neurovirol 22:170-8

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