The Core Laboratory will be directed by an experienced postdoctoral fellow. Dr. Jincun Zhao, under the guidance of the Core Leader, Dr. Perlman. The Core will be primarily based in a BSL3 laboratory that is equipped for tissue culture and animal work. Personnel working in the Core are experienced in virological and cell culture techniques. The Animal Core has several functions that are critical for success of this POl grant. It will propagate and titer nonrecombinant and recombinant mouse-adapted and Urbani strains of SARS-CoV and will infect mice with these viruses. It will monitor mice for clinical disease and weight loss and will harvest tissue. The Core will prepare samples for histological and immunohistochemical analysis and will prepare RNA and protein from infected tissue. The Core will analyze mice for antibody and T cell responses. The Core will also be responsible for ensuring that protocols for working with animals and for working under BSL3 laboratory conditions are up-to-date. While the Core will teach members of the Project methods important for analysis of mice under BSL3 conditions, it will primarily be responsible for performing most of these analyses. The Core will provide reagents and perform common assays efficiently, thereby standardizing results and enhancing synergistic interactions. Additionally, all projects will interact with the Core, thereby facilitating interactions between members of the PPG. By providng these services, the Core will allow Program investigators to focus on issues related to SARS-CoV pathogenesis and to the development of anti-viral therapies and vaccines.
This P01 grant is focused on understanding SARS-CoV pathogenesis and on developing novel anti-SARSCoV vaccines and therapies. The Animal Core will serve as a central facility for analyzing mice infected with SARS-CoV and for providing virus to the projects. By standardizing methodologies, it will facilitate data sharing among the projects and enhance synergistic interactions.
|Zhao, Jincun; Li, Kun; Wohlford-Lenane, Christine et al. (2014) Rapid generation of a mouse model for Middle East respiratory syndrome. Proc Natl Acad Sci U S A 111:4970-5|
|Usme-Ciro, Jose A; Lopera, Jaime A; Enjuanes, Luis et al. (2014) Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome. Virus Res 180:12-22|
|Barlan, Arlene; Zhao, Jincun; Sarkar, Mayukh K et al. (2014) Receptor variation and susceptibility to Middle East respiratory syndrome coronavirus infection. J Virol 88:4953-61|
|Nieto-Torres, Jose L; DeDiego, Marta L; Verdiá-Báguena, Carmina et al. (2014) Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis. PLoS Pathog 10:e1004077|
|DeDiego, Marta L; Nieto-Torres, Jose L; Regla-Nava, Jose A et al. (2014) Inhibition of NF-*B-mediated inflammation in severe acute respiratory syndrome coronavirus-infected mice increases survival. J Virol 88:913-24|
|Channappanavar, Rudragouda; Fett, Craig; Zhao, Jincun et al. (2014) Virus-specific memory CD8 T cells provide substantial protection from lethal severe acute respiratory syndrome coronavirus infection. J Virol 88:11034-44|
|DeDiego, Marta L; Nieto-Torres, Jose L; Jimenez-Guardeño, Jose M et al. (2014) Coronavirus virulence genes with main focus on SARS-CoV envelope gene. Virus Res 194:124-37|
|Almazan, Fernando; DeDiego, Marta L; Sola, Isabel et al. (2013) Engineering a replication-competent, propagation-defective Middle East respiratory syndrome coronavirus as a vaccine candidate. MBio 4:e00650-13|
|Regla-Nava, Jose A; Jimenez-Guardeno, Jose M; Nieto-Torres, Jose L et al. (2013) The replication of a mouse adapted SARS-CoV in a mouse cell line stably expressing the murine SARS-CoV receptor mACE2 efficiently induces the expression of proinflammatory cytokines. J Virol Methods 193:639-46|
|Gallagher, Tom; Perlman, Stanley (2013) Public health: Broad reception for coronavirus. Nature 495:176-7|
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