instmctions): CORE B: Cell and Tissue Acquisition Core Abstract: The purpose of this Core is to provide cellular material, DNA/RNA, serum, plasma, cell lines pathology specimens as available, and other materials to each investigator and to provide flow cytometry and cell sorting services in a timely fashion. We have an active laboratory that is well versed in cell isolation, storage, generation of cell lines, cell analyses, RNA and DNA isolation. While fresh samples are often needed for work in the Program project, this Core has ample storage facilities in liquid nitrogen and a log system for these materials so that they can be retrieved for later studies as needed. There is also an active flow cytometry facility with state ofthe art equipment for analysis and sorting. For tissue specimens there are also three sources of materials - the out patient offices, the operating rooms for surgical procedures and the endoscopy suite for gastrointestinal endoscopic biopsies. The P.l. (Dr. Mayer) has been working closely with Dr. Harpaz in Pathology, to secure surgical specimens for research. This has facilitated access to such tissues as well as input from the pathologists in terms of histologic analyses. This cell isolation, phenotyping, cell culture and tissue Core serves to support Projects 1-4 in this ongoing Program Project.

Public Health Relevance

As the work in this Program Project depends on rare materials, the Cell and Tissue Acquisition Core B is required to access, validate, isolate, maintain, record and distribute cells, DNA/RNA, serum, plasma, cell lines, pathology and other rare patient materials, so that research work can be carried out by each Project in the most expeditious manner.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1-PA-I)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Icahn School of Medicine at Mount Sinai
New York
United States
Zip Code
Casanova, Jean-Laurent; Abel, Laurent (2017) Human genetics of infectious diseases: Unique insights into immunological redundancy. Semin Immunol :
Boisson, Bertrand; Puel, Anne; Picard, Capucine et al. (2017) Human I?B? Gain of Function: a Severe and Syndromic Immunodeficiency. J Clin Immunol 37:397-412
Gernez, Yael; Freeman, Alexandra F; Holland, Steven M et al. (2017) Autosomal Dominant Hyper-IgE Syndrome in the USIDNET Registry. J Allergy Clin Immunol Pract :
Schickel, Jean-Nicolas; Glauzy, Salomé; Ng, Yen-Shing et al. (2017) Self-reactive VH4-34-expressing IgG B cells recognize commensal bacteria. J Exp Med 214:1991-2003
Perkins, Tiffany; Rosenberg, Jacob M; Le Coz, Carole et al. (2017) Smith-Magenis Syndrome Patients Often Display Antibody Deficiency but Not Other Immune Pathologies. J Allergy Clin Immunol Pract 5:1344-1350.e3
Ma, Chi A; Stinson, Jeffrey R; Zhang, Yuan et al. (2017) Germline hypomorphic CARD11 mutations in severe atopic disease. Nat Genet 49:1192-1201
Magri, Giuliana; Comerma, Laura; Pybus, Marc et al. (2017) Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals. Immunity 47:118-134.e8
Albin-Leeds, Stephanie; Ochoa, Juliana; Mehta, Harshna et al. (2017) Idiopathic T cell lymphopenia identified in New York State Newborn Screening. Clin Immunol 183:36-40
Di Niro, R; Snir, O; Kaukinen, K et al. (2016) Responsive population dynamics and wide seeding into the duodenal lamina propria of transglutaminase-2-specific plasma cells in celiac disease. Mucosal Immunol 9:254-64
Scott, Eric M; Halees, Anason; Itan, Yuval et al. (2016) Characterization of Greater Middle Eastern genetic variation for enhanced disease gene discovery. Nat Genet 48:1071-6

Showing the most recent 10 out of 153 publications