instmctions): CORE B: Cell and Tissue Acquisition Core Abstract: The purpose of this Core is to provide cellular material, DNA/RNA, serum, plasma, cell lines pathology specimens as available, and other materials to each investigator and to provide flow cytometry and cell sorting services in a timely fashion. We have an active laboratory that is well versed in cell isolation, storage, generation of cell lines, cell analyses, RNA and DNA isolation. While fresh samples are often needed for work in the Program project, this Core has ample storage facilities in liquid nitrogen and a log system for these materials so that they can be retrieved for later studies as needed. There is also an active flow cytometry facility with state ofthe art equipment for analysis and sorting. For tissue specimens there are also three sources of materials - the out patient offices, the operating rooms for surgical procedures and the endoscopy suite for gastrointestinal endoscopic biopsies. The P.l. (Dr. Mayer) has been working closely with Dr. Harpaz in Pathology, to secure surgical specimens for research. This has facilitated access to such tissues as well as input from the pathologists in terms of histologic analyses. This cell isolation, phenotyping, cell culture and tissue Core serves to support Projects 1-4 in this ongoing Program Project.

Public Health Relevance

As the work in this Program Project depends on rare materials, the Cell and Tissue Acquisition Core B is required to access, validate, isolate, maintain, record and distribute cells, DNA/RNA, serum, plasma, cell lines, pathology and other rare patient materials, so that research work can be carried out by each Project in the most expeditious manner.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program Projects (P01)
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Special Emphasis Panel (ZAI1-PA-I)
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Icahn School of Medicine at Mount Sinai
New York
United States
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Gaschignard, Jean; Levy, Corinne; Chrabieh, Maya et al. (2014) Invasive pneumococcal disease in children can reveal a primary immunodeficiency. Clin Infect Dis 59:244-51
Gutzeit, Cindy; Nagy, Noemi; Gentile, Maurizio et al. (2014) Exosomes derived from Burkitt's lymphoma cell lines induce proliferation, differentiation, and class-switch recombination in B cells. J Immunol 192:5852-62
Maglione, Paul J; Ko, Huaibin M; Beasley, Mary B et al. (2014) Tertiary lymphoid neogenesis is a component of pulmonary lymphoid hyperplasia in patients with common variable immunodeficiency. J Allergy Clin Immunol 133:535-42
Castiello, Maria Carmina; Bosticardo, Marita; Pala, Francesca et al. (2014) Wiskott-Aldrich Syndrome protein deficiency perturbs the homeostasis of B-cell compartment in humans. J Autoimmun 50:42-50
Al-Herz, Waleed; Bousfiha, Aziz; Casanova, Jean-Laurent et al. (2014) Primary immunodeficiency diseases: an update on the classification from the international union of immunological societies expert committee for primary immunodeficiency. Front Immunol 5:162
Magri, Giuliana; Miyajima, Michio; Bascones, Sabrina et al. (2014) Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol 15:354-64
Maglione, Paul J; Overbey, Jessica R; Radigan, Lin et al. (2014) Pulmonary radiologic findings in common variable immunodeficiency: clinical and immunological correlations. Ann Allergy Asthma Immunol 113:452-9
Keller, M; Glessner, J; Resnick, E et al. (2014) Burden of copy number variation in common variable immunodeficiency. Clin Exp Immunol 177:269-71
Gutzeit, Cindy; Magri, Giuliana; Cerutti, Andrea (2014) Intestinal IgA production and its role in host-microbe interaction. Immunol Rev 260:76-85
Menard, Laurence; Cantaert, Tineke; Chamberlain, Nicolas et al. (2014) Signaling lymphocytic activation molecule (SLAM)/SLAM-associated protein pathway regulates human B-cell tolerance. J Allergy Clin Immunol 133:1149-61

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