instmctions): CORE B: Cell and Tissue Acquisition Core Abstract: The purpose of this Core is to provide cellular material, DNA/RNA, serum, plasma, cell lines pathology specimens as available, and other materials to each investigator and to provide flow cytometry and cell sorting services in a timely fashion. We have an active laboratory that is well versed in cell isolation, storage, generation of cell lines, cell analyses, RNA and DNA isolation. While fresh samples are often needed for work in the Program project, this Core has ample storage facilities in liquid nitrogen and a log system for these materials so that they can be retrieved for later studies as needed. There is also an active flow cytometry facility with state ofthe art equipment for analysis and sorting. For tissue specimens there are also three sources of materials - the out patient offices, the operating rooms for surgical procedures and the endoscopy suite for gastrointestinal endoscopic biopsies. The P.l. (Dr. Mayer) has been working closely with Dr. Harpaz in Pathology, to secure surgical specimens for research. This has facilitated access to such tissues as well as input from the pathologists in terms of histologic analyses. This cell isolation, phenotyping, cell culture and tissue Core serves to support Projects 1-4 in this ongoing Program Project.

Public Health Relevance

As the work in this Program Project depends on rare materials, the Cell and Tissue Acquisition Core B is required to access, validate, isolate, maintain, record and distribute cells, DNA/RNA, serum, plasma, cell lines, pathology and other rare patient materials, so that research work can be carried out by each Project in the most expeditious manner.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
5P01AI061093-11
Application #
8719914
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Icahn School of Medicine at Mount Sinai
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10029
Uzzan, Mathieu; Ko, Huaibin M; Mehandru, Saurabh et al. (2016) Gastrointestinal Disorders Associated with Common Variable Immune Deficiency (CVID) and Chronic Granulomatous Disease (CGD). Curr Gastroenterol Rep 18:17
Chorny, Alejo; Casas-Recasens, Sandra; Sintes, Jordi et al. (2016) The soluble pattern recognition receptor PTX3 links humoral innate and adaptive immune responses by helping marginal zone B cells. J Exp Med 213:2167-85
Massaad, Michel J; Zhou, Jia; Tsuchimoto, Daisuke et al. (2016) Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity. J Clin Invest 126:4219-4236
Scott, Eric M; Halees, Anason; Itan, Yuval et al. (2016) Characterization of Greater Middle Eastern genetic variation for enhanced disease gene discovery. Nat Genet 48:1071-6
Cols, Montserrat; Rahman, Adeeb; Maglione, Paul J et al. (2016) Expansion of inflammatory innate lymphoid cells in patients with common variable immune deficiency. J Allergy Clin Immunol 137:1206-1215.e6
Lee, Alison Joanne; Moncada-Vélez, Marcela; Picard, Capucine et al. (2016) Severe Mycobacterial Diseases in a Patient with GOF IκBα Mutation Without EDA. J Clin Immunol 36:12-5
Cantaert, Tineke; Schickel, Jean-Nicolas; Bannock, Jason M et al. (2016) Decreased somatic hypermutation induces an impaired peripheral B cell tolerance checkpoint. J Clin Invest 126:4289-4302
Kuehn, Hye Sun; Boisson, Bertrand; Cunningham-Rundles, Charlotte et al. (2016) Loss of B Cells in Patients with Heterozygous Mutations in IKAROS. N Engl J Med 374:1032-43
Bonilla, Francisco A; Barlan, Isil; Chapel, Helen et al. (2016) International Consensus Document (ICON): Common Variable Immunodeficiency Disorders. J Allergy Clin Immunol Pract 4:38-59
Morbach, Henner; Schickel, Jean-Nicolas; Cunningham-Rundles, Charlotte et al. (2016) CD19 controls Toll-like receptor 9 responses in human B cells. J Allergy Clin Immunol 137:889-898.e6

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